A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC
Chemical Communications, vol. 56, pp. 8631–8634
Abstract
A colourimetric high-throughput screening system was established for directed evolution of prodigiosin ligase PigC. The two-step system consists of a colony prescreening test and a subsequent photometric 96-well plate assay. Screening PigC epPCR libraries in Pseudomonas putida revealed a PigC variant that achieved a 2.9× increased yield of prodiginine derivatives.
Authors 6
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Stefanie Brands Aachen
Affiliation as printed
52074 Aachen
Germany
Lehrstuhl für Biotechnologie
RWTH Aachen University
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Forschungszentrum Jülich · Heinrich Heine University Düsseldorf
Affiliation as printed
52426 Jülich
Germany
Heinrich Heine University Düsseldorf located at Forschungszentrum Jülich
Institute of Bioorganic Chemistry
Stetternicher Forst
-
Forschungszentrum Jülich · Heinrich Heine University Düsseldorf
Affiliation as printed
52426 Jülich
Germany
Heinrich Heine University Düsseldorf located at Forschungszentrum Jülich
Institute of Bioorganic Chemistry
Stetternicher Forst
-
Forschungszentrum Jülich · Heinrich Heine University Düsseldorf
Affiliation as printed
52426 Jülich
Germany
Heinrich Heine University Düsseldorf located at Forschungszentrum Jülich
Institute of Bioorganic Chemistry
Stetternicher Forst
-
Anna Joëlle Ruff Aachen
Affiliation as printed
52074 Aachen
Germany
Lehrstuhl für Biotechnologie
RWTH Aachen University
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DWI – Leibniz Institute for Interactive Materials · RWTH Aachen University
Affiliation as printed
52074 Aachen
DWI-Leibniz Institut für Interaktive Materialien
Germany
Lehrstuhl für Biotechnologie
RWTH Aachen University
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