Optimized prodigiosin production with Pseudomonas putida KT2440 using parallelized noninvasive online monitoring

Biotechnology Progress, vol. 38, pp. e3245

Abstract

The red pigment prodigiosin is of high pharmaceutical interest, due to its potential applications as an antitumor drug and antibiotic agent. As previously demonstrated, Pseudomonas putida KT2440 is a suitable host for prodigiosin production, as it exhibits high tolerance toward the antimicrobial properties of prodigiosin. So far, prodigiosin concentrations of up to 94 mg/L have been achieved in shake flask cultivations. For the characterization and optimization of the prodigiosin production process, the scattered light of P. putida and fluorescence of prodigiosin was measured. The excitation and emission wavelengths for prodigiosin measurement were analyzed by recording 2D fluorescence spectra. The strongest prodigiosin fluorescence was obtained at a wavelength combination of 535/560 nm. By reducing the temperature to 18 °C and using 16 g/L glucose, the prodigiosin concentration was more than doubled compared with the initial cultivation conditions. The obtained results demonstrate the capabilities of parallelized microscale cultivations combined with noninvasive online monitoring of fluorescence for rapid bioprocess development, using prodigiosin as a molecule of current biotechnological interest.

Authors 7

1. Carl Brehl Aachen AVT‑Biochemical Engineering

   Forschungszentrum Jülich · RWTH Aachen University

   Affiliation as printed

   AVT—Biochemical Engineering RWTH Aachen University Aachen Germany

   Bioeconomy Science Center (BioSC) Forschungszentrum Jülich GmbH Jülich Germany

2. Hannah U. C. Brass

   Forschungszentrum Jülich · Heinrich Heine University Düsseldorf

   Affiliation as printed

   Bioeconomy Science Center (BioSC) Forschungszentrum Jülich GmbH Jülich Germany

   Institute of Bioorganic Chemistry Heinrich Heine University Düsseldorf located at Forschungszentrum Jülich Jülich Germany

3. Clara Lüchtrath Aachen AVT‑Biochemical Engineering

   RWTH Aachen University

   Affiliation as printed

   AVT—Biochemical Engineering RWTH Aachen University Aachen Germany

4. Nina Ihling Aachen AVT‑Biochemical Engineering

   Forschungszentrum Jülich · RWTH Aachen University

   Affiliation as printed

   AVT—Biochemical Engineering RWTH Aachen University Aachen Germany

   Bioeconomy Science Center (BioSC) Forschungszentrum Jülich GmbH Jülich Germany

5. Thomas Claßen

   Forschungszentrum Jülich

   Affiliation as printed

   Bioeconomy Science Center (BioSC) Forschungszentrum Jülich GmbH Jülich Germany

   Institute for Bio‐ and Geosciences 1: Bioorganic Chemistry (IBG‐1) Forschungszentrum Jülich GmbH Jülich Germany

6. Jörg Pietruszka

   Forschungszentrum Jülich · Heinrich Heine University Düsseldorf

   Affiliation as printed

   Bioeconomy Science Center (BioSC) Forschungszentrum Jülich GmbH Jülich Germany

   Institute for Bio‐ and Geosciences 1: Bioorganic Chemistry (IBG‐1) Forschungszentrum Jülich GmbH Jülich Germany

   Institute of Bioorganic Chemistry Heinrich Heine University Düsseldorf located at Forschungszentrum Jülich Jülich Germany

7. Jochen Büchs corresponding Aachen AVT‑Biochemical Engineering

   Forschungszentrum Jülich · RWTH Aachen University

   Affiliation as printed

   AVT—Biochemical Engineering RWTH Aachen University Aachen Germany

   Bioeconomy Science Center (BioSC) Forschungszentrum Jülich GmbH Jülich Germany