Genome-Wide Scans and Transcriptomic Analyses Characterize Selective Changes as a Result of Chlorantraniliprole Resistance in Plutella xylostella
International Journal of Molecular Sciences, vol. 23, pp. 12245
Abstract
Pesticide resistance in insects is an example of adaptive evolution occurring in pest species and is driven by the artificial introduction of pesticides. The diamondback moth (DBM), Plutella xylostella (Lepidoptera: Plutellidae), has evolved resistance to various insecticides. Understanding the genetic changes underpinning the resistance to pesticides is necessary for the implementation of pest control measures. We sequenced the genome of six resistant and six susceptible DBM individuals separately and inferred the genomic regions of greatest divergence between strains using FST and θπ. Among several genomic regions potentially related to insecticide resistance, CYP6B6-like was observed with significant divergence between the resistant and susceptible strains, with a missense mutation located near the substrate recognition site (SRS) and four SNPs in the promoter. To characterize the relative effects of directional selection via insecticide tolerance (‘strain’) as compared to acute exposure to insecticide (‘treatment’), four pairwise comparisons were carried out between libraries to determine the differentially expressed genes. Most resistance-related differentially expressed genes were identified from the comparison of the strains and enriched in pathways for exogenous detoxification including cytochrome P450 and the ABC transporter. Further confirmation came from the weighted gene co-expression network analysis, which indicated that genes in the significant module associated with chlorantraniliprole resistance were enriched in pathways for exogenous detoxification, and that CYP6B6-like represented a hub gene in the “darkred” module. Furthermore, RNAi knock-down of CYP6B6-like increases P. xylostella sensitivity to chlorantraniliprole. Our study thus provides a genetic foundation underlying selection for pesticide resistance and plausible mechanisms to explain fast evolved adaptation through genomic divergence and altered gene expression in insects.
Authors 9
-
Affiliation as printed
Department of Grassland Resources and Ecology, College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China
-
Affiliation as printed
College of Plant Protection, China Agricultural University, Beijing 100193, China
-
Affiliation as printed
Naturalis Biodiversity Center, Understanding Evolution Group, The Natural History Museum, 2333 CR Leiden, The Netherlands
-
Affiliation as printed
College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
-
Affiliation as printed
Department of Grassland Resources and Ecology, College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China
-
Affiliation as printed
Department of Genetics, Evolution & Environment, University College London, London WC1E 6BT, UK
-
Affiliation as printed
College of Plant Protection, China Agricultural University, Beijing 100193, China
-
Affiliation as printed
Department of Agriculture, The University of Swabi, Anbar-Swabi 23561, Khyber Pakhtunkhwa, Pakistan
-
Liping Ban corresponding
Affiliation as printed
Department of Grassland Resources and Ecology, College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China
Cited by 2 stored of 2
2 results
No patents citing this paper on Lens.org (checked 2026-10-11).