Single‐cell omics: Overview, analysis, and application in biomedical science

Journal of Cellular Biochemistry, vol. 122, pp. 1571–1578

Abstract

Single-cell sequencing methods provide the highest resolution insight into cellular heterogeneity. Owing to their rapid growth and decreasing cost, they are now widely accessible to scientists worldwide. Single-cell technologies enable analysis of a large number of cells, making them powerful tools to characterise rare cell types and refine our understanding of diverse cell states. Moreover, single-cell application in biomedical sciences helps to unravel mechanisms related to disease pathogenesis and outcome. In this Viewpoint, we briefly describe existing single-cell methods (genomics, transcriptomics, epigenomics, proteomics, and mulitomics), comment on available analysis tools, and give examples of method applications in the biomedical field.

Authors 4

1. Catarina M. Stein

   Charité - Universitätsmedizin Berlin

   Affiliation as printed

   Berlin School Of Integrative Oncology (BSIO) Charité—Universitätsmedizin Berlin Berlin Germany

   Department of Hematology, Oncology, and Tumor Immunology Charité—Universitätsmedizin Berlin Berlin Germany

2. Ralf Weiskirchen Aachen Institute of Molecular Pathobiochemistry Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC)

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC) RWTH University Hospital Aachen Aachen Germany

   Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), RWTH University Hospital Aachen, Aachen, Germany

3. Frédérik Damm

   Berlin Institute of Health at Charité - Universitätsmedizin Berlin · Deutsches Konsortium für Translationale Krebsforschung · Charité - Universitätsmedizin Berlin

   Affiliation as printed

   Berlin Institute of Health (BIH) Berlin Germany

   Department of Hematology, Oncology, and Tumor Immunology Charité—Universitätsmedizin Berlin Berlin Germany

   German Consortium for Translational Cancer Research (DKTK) Heidelberg Germany

   German Consortium for Translational Cancer Research (DKTK), Heidelberg, Germany

4. Paulina M. Strzelecka corresponding

   Deutsches Konsortium für Translationale Krebsforschung · Charité - Universitätsmedizin Berlin

   Affiliation as printed

   Department of Hematology, Oncology, and Tumor Immunology Charité—Universitätsmedizin Berlin Berlin Germany

   German Consortium for Translational Cancer Research (DKTK) Heidelberg Germany