Visualizing the codevelopment of structural and immune cells in the human fetal lung using imaging mass cytometry

American Journal of Physiology-Lung Cellular and Molecular Physiology, vol. 330, pp. L77–L86

Abstract

This study provides a detailed protocol to visualize the codevelopment of structural and immune cells in the human fetal lung through imaging mass cytometry (IMC). Using IMC, spatial relationships between lung epithelial cells and myeloid cells can be visualized, which may contribute to unraveling cellular interactions important for lung maturation.

Authors 7

1. Jia Li Aachen PulmoScience Lab Department of Immunology, Leiden University Medical Center

   Leiden University Medical Center · State Key Laboratory of Respiratory Disease · Guangzhou Institute of Respiratory Health · Guangzhou Medical University

   Affiliation as printed

   Leiden University Medical Center (LUMC)

   National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, Guangzhou Institute of Respiratory Health, Hospital of Guangzhou Medical University

   PulmoScience Lab, Leiden University Medical Center (LUMC)

   Department of Immunology, Leiden University Medical Center (LUMC), Leiden, The Netherlands

   State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, Guangzhou Institute of Respiratory Health, Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China

2. Susana M. Chuva de Sousa Lopes Aachen Department of Anatomy and Embryology

   Leiden University Medical Center

   Affiliation as printed

   Leiden University Medical Center (LUMC)

   Department of Anatomy and Embryology, Leiden University Medical Center (LUMC), Leiden, The Netherlands

3. Vincent van Unen Aachen Department of Immunology, Leiden University Medical Center

   Leiden University Medical Center

   Affiliation as printed

   Leiden University Medical Center (LUMC)

   Department of Immunology, Leiden University Medical Center (LUMC), Leiden, The Netherlands

4. Pieter Sicco Hiemstra Aachen PulmoScience Lab Department of Pulmonology, PulmoScience Lab

   Leiden University Medical Center

   Affiliation as printed

   PulmoScience Lab, Leiden University Medical Center (LUMC)

   Department of Pulmonology, PulmoScience Lab, Leiden University Medical Center (LUMC), Leiden, The Netherlands

5. Jan N Stolk Aachen PulmoScience Lab Department of Pulmonology, PulmoScience Lab

   Leiden University Medical Center

   Affiliation as printed

   PulmoScience Lab, Leiden University Medical Center (LUMC)

   Department of Pulmonology, PulmoScience Lab, Leiden University Medical Center (LUMC), Leiden, The Netherlands

6. Frits Koning Aachen Department of Immunology, Leiden University Medical Center

   Leiden University Medical Center

   Affiliation as printed

   Leiden University Medical Center (LUMC)

   Department of Immunology, Leiden University Medical Center (LUMC), Leiden, The Netherlands

7. Padmini P. S. J. Khedoe Aachen PulmoScience Lab Department of Pulmonology, PulmoScience Lab

   Leiden University Medical Center

   Affiliation as printed

   PulmoScience Lab, Leiden University Medical Center (LUMC)

   Department of Pulmonology, PulmoScience Lab, Leiden University Medical Center (LUMC), Leiden, The Netherlands