FadA antigen of Fusobacterium nucleatum: implications for ceRNA network in colorectal cancer and adenomatous polyps progression

Discover Oncology, vol. 16, pp. 58

Abstract

INTRODUCTION: Colorectal cancer (CRC) is the second most common cause of cancer-related deaths globally. The gut microbiota, along with adenomatous polyps (AP), has emerged as a plausible contributor to CRC progression. This study aimed to scrutinize the impact of the FadA antigen derived from Fusobacterium nucleatum on the expression levels of the ANXA2 ceRNA network and assess its relevance to CRC advancement. MATERIAL AND METHODS: The functions of ANXA2 and LINC00460 in CRC have been partially clarified. According to our previous study to identify shared MicroRNA-Interaction-Targets (MITs) between ANXA2 and LINC00460, TargetScanHuman (V7.2) and miRDB databases have been used respectively. The Bioinformatics and Evolutionary Genomics web tool was employed to intersect the sets of shared microRNAs and their common targets. Then, the ANXA2 ceRNA network was constructed. Subsequently, the mRNA, miRNA, and lncRNA expression levels were examined in intestinal biopsy specimens from 30 healthy controls, 30 Adenoma patients, and 30 cases of CRC stage I using qRT-PCR. RESULTS: = 0.9311, p < 0.0001). Also, the functional analysis of ANXA2 revealed its impact on CRC progression through JAK-STAT and Hippo signaling pathways. CONCLUSION: FadA appears to potentiate CRC progression by inducing the upregulation of LINC00460, consequently leading to the hyperexpression of ANXA2 through the ceRNA network.

Authors 10

1. Sama Rezasoltani Aachen Division of Oral Microbiology and Immunology Department of Operative Dentistry

   RWTH Aachen University · Universitätsklinikum Aachen

   Affiliation as printed

   Division of Oral Microbiology and Immunology, Department of Operative Dentistry, Periodontology and Preventive Dentistry, RWTH University Hospital, Aachen, Germany

2. Elahe Shams

   Shahid Beheshti University of Medical Sciences · Research Institute for Gastroenterology and Liver Diseases

   Affiliation as printed

   Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3. Moein Piroozkhah

   Shahid Beheshti University of Medical Sciences · Research Institute for Gastroenterology and Liver Diseases

   Affiliation as printed

   Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

4. Yaser Aidi

   Islamic Azad University Central Tehran Branch

   Affiliation as printed

   Department of Genetics, Islamic Azad University of Central Tehran Branch, Tehran, Iran

5. Mehdi Azizmohammad Looha

   Shahid Beheshti University of Medical Sciences · Research Institute for Gastroenterology and Liver Diseases

   Affiliation as printed

   Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

6. Parmida Bagheri

   Shahid Beheshti University

   Affiliation as printed

   Department of Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

7. Roudabeh Behzadi Andouhjerdi

   Islamic Azad University Central Tehran Branch

   Affiliation as printed

   Department of Genetics, Islamic Azad University of Central Tehran Branch, Tehran, Iran

8. Amir Sadeghi

   Shahid Beheshti University of Medical Sciences · Research Institute for Gastroenterology and Liver Diseases

   Affiliation as printed

   Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

9. Leili Rejali corresponding

   Shahid Beheshti University · Shahid Beheshti University of Medical Sciences · Research Institute for Gastroenterology and Liver Diseases

   Affiliation as printed

   Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran. leilirejali@gmail.com

   Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

10. Ehsan Nazemalhosseini Mojarad corresponding

    Shahid Beheshti University · Leiden University Medical Center · Shahid Beheshti University of Medical Sciences · Research Institute for Gastroenterology and Liver Diseases

    Affiliation as printed

    Department of Surgery, Leiden University Medical Center, Leiden, Netherlands. ehsanmojarad@gmail.com

    Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran. ehsanmojarad@gmail.com

    Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran