Proteasome composition in immune cells implies special immune‐cell‐specific immunoproteasome function

European Journal of Immunology, vol. 54, pp. e2350613

Abstract

Abstract Immunoproteasomes are a special class of proteasomes, which can be induced with IFN‐γ in an inflammatory environment. In recent years, it became evident that certain immune cell types constitutively express high levels of immunoproteasomes. However, information regarding the basal expression of proteolytically active immunoproteasome subunits in different types of immune cells is still rare. Hence, we quantified standard proteasome subunits (β1c, β2c, β5c) and immunoproteasome subunits (LMP2, MECL‐1, LMP7) in the major murine (CD4 + T cells, CD8 + T cells, CD19 + B cells, CD11c + dendritic cells, CD49d + natural killer cells, Ly‐6G + neutrophils) and human immune cell (CD4 + T cells, CD8 + T cells, CD19 + B cells, CD1c + CD141 + myeloid dendritic cells, CD56 + natural killer cells, granulocytes) subsets. The different human immune cell types were isolated from peripheral blood and the murine immune cell subsets from spleen. We found that proteasomes of most immune cell subsets mainly consist of immunoproteasome subunits. Our data will serve as a reference and guideline for immunoproteasome expression and imply a special role of immunoproteasomes in immune cells.

Authors 8

1. Katharina Inholz

   University of Konstanz · Institute of Cell Biology and Immunology Thurgau

   Affiliation as printed

   Biotechnology Institute Thurgau (BITg) at the University of Konstanz Kreuzlingen Switzerland

   Division of Immunology Department of Biology University of Konstanz Konstanz Germany

   Biotechnology Institute Thurgau (BITg) at the University of Konstanz, Kreuzlingen, Switzerland

   Division of Immunology, Department of Biology, University of Konstanz, Konstanz, Germany

2. Janet L. Anderl
   Affiliation as printed

   Department of Research Kezar Life Sciences South San Francisco California USA

   Department of Research, Kezar Life Sciences, South San Francisco, California, USA

3. Moritz Klawitter

   University of Konstanz

   Affiliation as printed

   Division of Immunology Department of Biology University of Konstanz Konstanz Germany

   Division of Immunology, Department of Biology, University of Konstanz, Konstanz, Germany

4. Heike Goebel

   University of Konstanz

   Affiliation as printed

   Division of Immunology Department of Biology University of Konstanz Konstanz Germany

   Division of Immunology, Department of Biology, University of Konstanz, Konstanz, Germany

5. Elmer Maurits Aachen

   Leiden University

   Affiliation as printed

   Leiden Institute of Chemistry Leiden University Leiden the Netherlands

   Leiden Institute of Chemistry, Leiden University, Leiden, the Netherlands

6. Christopher J. Kirk
   Affiliation as printed

   Department of Research Kezar Life Sciences South San Francisco California USA

   Department of Research, Kezar Life Sciences, South San Francisco, California, USA

7. Rong Fan
   Affiliation as printed

   Department of Research Kezar Life Sciences South San Francisco California USA

   Department of Research, Kezar Life Sciences, South San Francisco, California, USA

8. Michael Basler corresponding

   University of Konstanz · Institute of Cell Biology and Immunology Thurgau

   Affiliation as printed

   Biotechnology Institute Thurgau (BITg) at the University of Konstanz Kreuzlingen Switzerland

   Division of Immunology Department of Biology University of Konstanz Konstanz Germany

   Biotechnology Institute Thurgau (BITg) at the University of Konstanz, Kreuzlingen, Switzerland

   Division of Immunology, Department of Biology, University of Konstanz, Konstanz, Germany