Modeling iPSC-Derived Endothelial Cell Transition in Tumor Angiogenesis Using Petri Nets

International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC), pp. 337–346

Abstract

Tumor angiogenesis concerns the development of new blood vessels supplying the necessary nutrients for the further development of existing tumor cells. The entire process is complex, involving the production and consumption of chemicals, endothelial cell transitions as well as cell interactions, divisions, and migrations. Microfluidic cell culture platform has been used to study angiogenesis of endothelial cells derived from human induced pluripotent stem cells (iPSC-ECs) for a physiological relevant micro-environment. In this paper, we elaborate on how to define a pipeline for simulating the transformation and process that an iPSC-derived endothelial cell goes through in this biological scenario. We leverage the robustness and simplicity of Petri nets for modeling the cell transformation and associated constraints. The environmental and spacial factors are added using custom 2-dimensional grids. Although the pipeline does not capture the entire complexity of tumor angiogenesis, we are able to capture the essence of endothelial cell transitions in tumor angiogenesis using this conceptually simplified solution.

Authors 5

1. Adéla Šterberová Aachen Leiden Insisute of Advanced Computer Science

   Leiden University

   Affiliation as printed

   Leiden Insisute of Advanced Computer Science, Leiden University, Leiden, The Netherlands, --- Select a Country ---

2. Andreea Dincu Aachen Leiden Insisute of Advanced Computer Science

   Leiden University

   Affiliation as printed

   Leiden Insisute of Advanced Computer Science, Leiden University, Leiden, The Netherlands, --- Select a Country ---

3. Stijn Oudshoorn Aachen Leiden Insisute of Advanced Computer Science

   Leiden University

   Affiliation as printed

   Leiden Insisute of Advanced Computer Science, Leiden University, Leiden, The Netherlands, --- Select a Country ---

4. Vincent van Duinen Aachen

   Leiden University Medical Center

   Affiliation as printed

   Leiden University Medical Center, Leiden, The Netherlands, --- Select a Country ---

5. Lu Cao corresponding Aachen Leiden Insisute of Advanced Computer Science

   Leiden University

   Affiliation as printed

   Leiden Insisute of Advanced Computer Science, Leiden University, Leiden, The Netherlands, --- Select a Country ---