Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging
Nature Methods, vol. 20, pp. 1174–1178
Abstract
Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.
Authors 38
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Indiana University Bloomington · Indiana University
Affiliation as printed
Department of Intelligent Systems Engineering, Indiana University, Bloomington, IN, USA
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Vanderbilt University · Vanderbilt University Medical Center
Affiliation as printed
Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA
Vanderbilt Diabetes Center, Vanderbilt University School of Medicine, Nashville, TN, USA
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GE Global Research (United States)
Affiliation as printed
GE Research, Niskayuna, NY, USA
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Stanford Medicine · Stanford University
Affiliation as printed
Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA
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Medizinische Hochschule Hannover
Affiliation as printed
Institute of Pathology, Hannover Medical School, Hannover, Germany
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GE Global Research (United States)
Affiliation as printed
GE Research, Niskayuna, NY, USA
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Affiliation as printed
Department of Biological Sciences, Columbia University, New York, NY, USA
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Science for Life Laboratory · Stanford Medicine · KTH Royal Institute of Technology · Stanford University
Affiliation as printed
Department of Bioengineering, Stanford University, Stanford, CA, USA
Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA
Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden
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Affiliation as printed
Department of Chemistry, Pennsylvania State University, University Park, PA, USA
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GE Global Research (United States)
Affiliation as printed
GE Research, Niskayuna, NY, USA
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Vanderbilt University · University of California, Davis
Affiliation as printed
Department of Biochemistry, Vanderbilt University, Nashville, TN, USA
Department of Chemistry, University of California Davis, Davis, CA, USA
Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA
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Science for Life Laboratory · KTH Royal Institute of Technology
Affiliation as printed
Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden
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Affiliation as printed
Department of Chemistry, Columbia University, New York, NY, USA
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Affiliation as printed
Department of Business Development, BioLegend Inc., San Diego, CA, USA
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Affiliation as printed
Department of Research and Development, Abcam PLC, Discovery Drive, Cambridge Biomedical Campus, Cambridge, UK
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Cell Signaling Technology (United States)
Affiliation as printed
Department of Research and Development, Cell Signaling Technology, Inc., Danvers, MA, USA
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Thermo Fisher Scientific (United States)
Affiliation as printed
Department of Protein and Cell Analysis, Thermo Fisher Scientific, Carlsbad, CA, USA
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Akoya Biosciences (United States)
Affiliation as printed
Akoya Biosciences, Marlborough, MA, USA
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Affiliation as printed
Department of Biomedical Informatics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
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Affiliation as printed
Department of Biochemistry, Vanderbilt University, Nashville, TN, USA
Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, USA
Department of Chemistry, Vanderbilt University, Nashville, TN, USA
Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA
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University of Rochester Medicine · University of Rochester
Affiliation as printed
Department of Pediatrics, University of Rochester Medical Center, Rochester, NY, USA
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University of Washington · University of Washington Medical Center
Affiliation as printed
Department of Pathology, University of Washington Medical Center, Seattle, WA, USA
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GE Global Research (United States)
Affiliation as printed
GE Research, Niskayuna, NY, USA
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Stanford Medicine · Stanford University
Affiliation as printed
Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA
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Buck Institute for Research on Aging
Affiliation as printed
Buck Institute for Research on Aging, Novato, CA, USA
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Medizinische Hochschule Hannover · German Center for Lung Research · RWTH Aachen University
Affiliation as printed
German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany
Institute of Pathology, Hannover Medical School, Hannover, Germany
Institute of Pathology, RWTH University of Aachen, Aachen, Germany
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Affiliation as printed
Departments of Chemistry, Molecular Biosciences and the Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA
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Broad Institute · Beth Israel Deaconess Medical Center · Harvard University
Affiliation as printed
Broad Institute of MIT and Harvard, Cambridge, MA, USA
Department of Pathology, Beth Israel Deaconess Medical Center, Boston, MA, USA
Spatial Technologies Unit, Harvard Medical School Initiative for RNA Medicine, Beth Israel Deaconess Medical Center, Boston, MA, USA
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Miltenyi Biotec (Germany) · University of Manchester
Affiliation as printed
Division of Infection, Immunity and Respiratory Medicine, University of Manchester, Manchester, UK
Miltenyi Biotec B.V. and Co. KG, Bergisch Gladbach, Germany
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Affiliation as printed
Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA
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Affiliation as printed
Department of Biological Sciences, Columbia University, New York, NY, USA
Department of Chemistry, Columbia University, New York, NY, USA
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Science for Life Laboratory · Stanford Medicine · KTH Royal Institute of Technology · Stanford University
Affiliation as printed
Department of Bioengineering, Stanford University, Stanford, CA, USA
Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA
Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden
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Stanford Medicine · Stanford University
Affiliation as printed
Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Laboratory of Immune System Biology, Lymphocyte Biology Section and Center for Advanced Tissue Imaging, NIAID, NIH, Bethesda, MD, USA
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Affiliation as printed
Departments of Chemistry, Molecular Biosciences and the Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA
Inotiv, Nashville, TN, USA
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Affiliation as printed
Departments of Chemistry, Molecular Biosciences and the Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA
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Indiana University Bloomington · Indiana University
Affiliation as printed
Department of Intelligent Systems Engineering, Indiana University, Bloomington, IN, USA
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Andrea J. Radtke corresponding
National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Laboratory of Immune System Biology, Lymphocyte Biology Section and Center for Advanced Tissue Imaging, NIAID, NIH, Bethesda, MD, USA. andrea.radtke@nih.gov
Laboratory of Immune System Biology, Lymphocyte Biology Section and Center for Advanced Tissue Imaging, NIAID, NIH, Bethesda, MD, USA
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