Quantify permeability using on-a-chip models in high-throughput applications
STAR Protocols, vol. 4, pp. 102051
Abstract
Traditionally, to quantify permeability of a biological barrier, the initial slope is used, based on the assumption of sink condition (concentration of the donor is constant, and the receiver increases less than 10%). With on-a-chip barrier models, this assumption fails in cell-free or leaky conditions, which requires the use of the exact solution. To encounter a time delay from performing the assay and acquiring the data, we present a protocol with the exact equation modified to incorporate a time offset.
Authors 8
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Camilla Soragni corresponding
Affiliation as printed
MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands; Department of Cardiology, Maastricht University, P. Debyelaan 25, 6229 HX Maastricht, the Netherlands. Electronic address: c.soragni@maastrichtuniversity.nl
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Affiliation as printed
MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands
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Affiliation as printed
MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands
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Affiliation as printed
MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands; Department of Cardiology, Maastricht University, P. Debyelaan 25, 6229 HX Maastricht, the Netherlands
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Affiliation as printed
MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands
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Affiliation as printed
MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands
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Affiliation as printed
Department of Cardiology, Maastricht University, P. Debyelaan 25, 6229 HX Maastricht, the Netherlands
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Affiliation as printed
MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands. Electronic address: c.ng@mimetas.com
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