Protocol for a placenta-on-a-chip model using trophoblasts differentiated from human induced pluripotent stem cells

STAR Protocols, vol. 5, pp. 102879

Abstract

Organ-on-a-chip technology incorporating stem cell techniques represents a promising strategy to improve modeling of human organs. Here, we present a protocol for generating a standardized 3D placenta-on-a-chip model using trophoblast derived from human induced pluripotent stem cells (hiPSCs). We describe steps for seeding hiPSCs into multi-chip OrganoPlate devices and on-chip differentiation into trophoblasts against an extracellular matrix under perfused conditions. We then detail procedures for conducting a functional barrier integrity assay, immunostaining, and collecting protein or RNA for molecular analysis. For complete details on the use and execution of this protocol, please refer to Lermant et al. (2023).1

Authors 5

1. Agathe Lermant

   University of Dundee

   Affiliation as printed

   Systems Medicine, School of Medicine, University of Dundee, DD1 9SY Dundee, UK

2. Gwenaëlle Rabussier Aachen

   Mimetas (Netherlands)

   Affiliation as printed

   MIMETAS, 2342 DH, Oegstgeest, the Netherlands

3. Lindsey Davidson

   University of Dundee

   Affiliation as printed

   Human Pluripotent Stem Cell Facility, School of Life Sciences, University of Dundee, Dundee, UK

4. Henriëtte L. Lanz Aachen

   Mimetas (Netherlands)

   Affiliation as printed

   MIMETAS, 2342 DH, Oegstgeest, the Netherlands

5. Colin E. Murdoch corresponding

   University of Dundee

   Affiliation as printed

   Systems Medicine, School of Medicine, University of Dundee, DD1 9SY Dundee, UK. Electronic address: c.z.murdoch@dundee.ac.uk

   Systems Medicine, School of Medicine, University of Dundee, DD1 9SY Dundee, UK