SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection
Experimental & Molecular Medicine, vol. 54, pp. 2188–2199
Abstract
The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories. SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.
Authors 16
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Szu-Hsien Sam Wu corresponding
Institute of Molecular Biotechnology · University of Vienna · Austrian Academy of Sciences · Vienna Biocenter · Medical University of Vienna
Affiliation as printed
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria. samwu202@gmail.com
Vienna BioCenter PhD Program, Doctoral School of the University at University of Vienna and Medical University of Vienna, 1030, Vienna, Austria. samwu202@gmail.com
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria
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Institute of Molecular Biotechnology · Austrian Academy of Sciences · Institute for Basic Science · Vienna Biocenter
Affiliation as printed
Center for Genome Engineering, Institute for Basic Science, Expo-ro 55, Yuseong-gu, Daejeon, 34126, Republic of Korea
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria
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Institute of Molecular Biotechnology · Austrian Academy of Sciences · Vienna Biocenter
Affiliation as printed
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria
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Institute of Molecular Biotechnology · Austrian Academy of Sciences · Vienna Biocenter
Affiliation as printed
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria
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Institute of Molecular Biotechnology · Austrian Academy of Sciences · Vienna Biocenter
Affiliation as printed
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria
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University of Vienna · Research Institute of Molecular Pathology · Vienna Biocenter · Medical University of Vienna
Affiliation as printed
Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Campus-Vienna-BioCenter 1, 1030, Vienna, Austria
Vienna BioCenter PhD Program, Doctoral School of the University at University of Vienna and Medical University of Vienna, 1030, Vienna, Austria
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Institute of Molecular Biotechnology · Austrian Academy of Sciences · Vienna Biocenter
Affiliation as printed
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria
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Institute of Molecular Biotechnology · Austrian Academy of Sciences · Institute for Basic Science · Vienna Biocenter
Affiliation as printed
Center for Genome Engineering, Institute for Basic Science, Expo-ro 55, Yuseong-gu, Daejeon, 34126, Republic of Korea
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria
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Institute of Molecular Biotechnology · Austrian Academy of Sciences · Vienna Biocenter · Catholic University of Korea
Affiliation as printed
Department of Medical and Biological Sciences, Catholic University of Korea, Bucheon, 14662, South Korea
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria
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Institute of Molecular Biotechnology · Austrian Academy of Sciences · Vienna Biocenter
Affiliation as printed
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria
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Leiden University Medical Center
Affiliation as printed
Transgenic Facility Leiden, Central Animal Facility, Leiden University Medical Center, Postbus 9600, 2300 RC, Leiden, The Netherlands
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Institute of Molecular Biotechnology · Austrian Academy of Sciences · Vienna Biocenter
Affiliation as printed
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria
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Leiden University Medical Center
Affiliation as printed
Transgenic Facility Leiden, Central Animal Facility, Leiden University Medical Center, Postbus 9600, 2300 RC, Leiden, The Netherlands
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Research Institute of Molecular Pathology
Affiliation as printed
IMP/IMBA Transgenic Service, Institute of Molecular Pathology (IMP), Vienna, Austria
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Research Institute of Molecular Pathology · Vienna Biocenter
Affiliation as printed
Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Campus-Vienna-BioCenter 1, 1030, Vienna, Austria
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Bon‐Kyoung Koo corresponding
Institute of Molecular Biotechnology · Austrian Academy of Sciences · Institute for Basic Science · Vienna Biocenter
Affiliation as printed
Center for Genome Engineering, Institute for Basic Science, Expo-ro 55, Yuseong-gu, Daejeon, 34126, Republic of Korea. koobk@ibs.re.kr
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria. koobk@ibs.re.kr
Center for Genome Engineering, Institute for Basic Science, Expo-ro 55, Yuseong-gu, Daejeon, 34126, Republic of Korea
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