Capillary microsampling-based single-cell metabolomics by mass spectrometry and its applications in medicine and drug discovery

Cancer Biomarkers, vol. 33, pp. 437–447

Abstract

Characterization of cellular metabolic states is a technical challenge in biomedicine. Cellular heterogeneity caused by inherent diversity in expression of metabolic enzymes or due to sensitivity of metabolic reactions to perturbations, necessitates single cell analysis of metabolism. Heterogeneity is typically seen in cancer and thus, single-cell metabolomics is expectedly useful in studying cancer progression, metastasis, and variations in cancer drug response. However, low sample volumes and analyte concentrations limit detection of critically important metabolites. Capillary microsampling-based mass spectrometry approaches are emerging as a promising solution for achieving single-cell omics. Herein, we focus on the recent advances in capillary microsampling-based mass spectrometry techniques for single-cell metabolomics. We discuss recent technical developments and applications to cancer medicine and drug discovery.

Authors 5

1. Yasmine Abouleila Aachen Division of Systems Biomedicine and Pharmacology Leiden Academic Centre for Drug Research Division of Sys-tems Biomedicine and Pharmacology

   Leiden University · RIKEN · RIKEN Center for Biosystems Dynamics Research · Misr International University

   Affiliation as printed

   Division of Systems Biomedicine and Pharmacology, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands

   Research Center, Misr International University, Cairo, Egypt

   Division of Sys-tems Biomedicine and Pharmacology, Leiden Academic Centre for Drug Research, Leiden University, Einsteinweg 55, 2333 CC Lei-den, The Netherlands

   Laboratory for Cell-free Protein Synthesis, RIKEN Center for Biosystems Dynamics Re-search (BDR), 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan

2. Ahmed Ali Aachen Division of Systems Biomedicine and Pharmacology Leiden Academic Centre for Drug Research Division of Sys-tems Biomedicine and Pharmacology

   Leiden University · RIKEN · RIKEN Center for Biosystems Dynamics Research · Misr International University

   Affiliation as printed

   Division of Systems Biomedicine and Pharmacology, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands

   Research Center, Misr International University, Cairo, Egypt

   Division of Sys-tems Biomedicine and Pharmacology, Leiden Academic Centre for Drug Research, Leiden University, Einsteinweg 55, 2333 CC Lei-den, The Netherlands

   Laboratory for Cell-free Protein Synthesis, RIKEN Center for Biosystems Dynamics Re-search (BDR), 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan

3. Keiko Masuda Aachen Division of Sys-tems Biomedicine and Pharmacology Leiden Academic Centre for Drug Research

   Leiden University · RIKEN · RIKEN Center for Biosystems Dynamics Research

   Affiliation as printed

   RIKEN Center for Biosystems Dynamics Research, Osaka, Japan

   Division of Sys-tems Biomedicine and Pharmacology, Leiden Academic Centre for Drug Research, Leiden University, Einsteinweg 55, 2333 CC Lei-den, The Netherlands

   Laboratory for Cell-free Protein Synthesis, RIKEN Center for Biosystems Dynamics Re-search (BDR), 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan

4. Alireza Mashaghi corresponding Aachen Division of Systems Biomedicine and Pharmacology Leiden Academic Centre for Drug Research

   Leiden University · RIKEN Center for Biosystems Dynamics Research · Misr International University

   Affiliation as printed

   Division of Systems Biomedicine and Pharmacology, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands

   RIKEN Center for Biosystems Dynamics Research, Osaka, Japan

   Research Center, Misr International University, Cairo, Egypt

5. Yoshihiro Shimizu corresponding Aachen Division of Systems Biomedicine and Pharmacology Leiden Academic Centre for Drug Research

   Leiden University · RIKEN Center for Biosystems Dynamics Research

   Affiliation as printed

   RIKEN Center for Biosystems Dynamics Research, Osaka, Japan

   Division of Systems Biomedicine and Pharmacology, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands