Initial properdin binding contributes to alternative pathway activation at the surface of viable and necrotic cells
European Journal of Immunology, vol. 52, pp. 597–608
Abstract
Properdin, the only known positive regulator of the complement system, stabilizes the C3 convertase, thereby increasing its half-life. In contrast to most other complement factors, properdin is mainly produced extrahepatically by myeloid cells. Recent data suggest a role for properdin as a pattern recognition molecule. Here, we confirmed previous findings of properdin binding to different necrotic cells including Jurkat T cells. Binding can occur independent of C3, as demonstrated by HAP-1 C3 KO cells, excluding a role for endogenous C3. In view of the cellular source of properdin, interaction with myeloid cells was examined. Properdin bound to the surface of viable monocyte-derived pro- and anti-inflammatory macrophages, but not to DCs. Binding was demonstrated for purified properdin as well as fractionated P2, P3, and P4 properdin oligomers. Binding contributed to local complement activation as determined by C3 and C5b-9 deposition on the cell surfaces and seems a prerequisite for alternative pathway activation. Interaction of properdin with cell surfaces could be inhibited with the tick protein Salp20 and by different polysaccharides, depending on sulfation and chain length. These data identify properdin as a factor interacting with different cell surfaces, being either dead or alive, contributing to the local stimulation of complement activation.
Authors 10
-
Mieke F. van Essen Aachen Division of Nephrology and Transplant Medicine Department of Medicine Leiden University Medical Center Leiden The Netherlands Division of Nephrology and Transplant Medicine Department of Medicine, Leiden University Medical Center
Leiden University Medical Center
Affiliation as printed
Division of Nephrology and Transplant Medicine Department of Medicine Leiden University Medical Center Leiden The Netherlands
Division of Nephrology and Transplant Medicine, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
-
Nicole Schlagwein Aachen Division of Nephrology and Transplant Medicine Department of Medicine Leiden University Medical Center Leiden The Netherlands Division of Nephrology and Transplant Medicine Department of Medicine, Leiden University Medical Center
Leiden University Medical Center
Affiliation as printed
Division of Nephrology and Transplant Medicine Department of Medicine Leiden University Medical Center Leiden The Netherlands
Division of Nephrology and Transplant Medicine, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
-
Elisa M.P. van den Hoven Aachen Division of Nephrology and Transplant Medicine Department of Medicine Leiden University Medical Center Leiden The Netherlands Division of Nephrology and Transplant Medicine Department of Medicine, Leiden University Medical Center
Leiden University Medical Center
Affiliation as printed
Division of Nephrology and Transplant Medicine Department of Medicine Leiden University Medical Center Leiden The Netherlands
Division of Nephrology and Transplant Medicine, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
-
Daniëlle J. van Gijlswijk‐Janssen Aachen Division of Nephrology and Transplant Medicine Department of Medicine Leiden University Medical Center Leiden The Netherlands Division of Nephrology and Transplant Medicine Department of Medicine, Leiden University Medical Center
Leiden University Medical Center
Affiliation as printed
Division of Nephrology and Transplant Medicine Department of Medicine Leiden University Medical Center Leiden The Netherlands
Division of Nephrology and Transplant Medicine, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
-
Rosalie Lubbers Aachen Department of Rheumatology Leiden University Medical Center Leiden The Netherlands Department of Rheumatology, Leiden University Medical Center
Leiden University Medical Center
Affiliation as printed
Department of Rheumatology Leiden University Medical Center Leiden The Netherlands
Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands
-
Affiliation as printed
Faculty of Science Department of Chemistry Crystal and Structural Chemistry Bijvoet Center for Biomolecular Research Utrecht University Utrecht The Netherlands
Faculty of Science, Department of Chemistry, Crystal and Structural Chemistry, Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands
-
University Medical Center Groningen
Affiliation as printed
Department of Nephrology University Medical Center Groningen Groningen The Netherlands
Department of Nephrology, University Medical Center Groningen, Groningen, The Netherlands
-
Jurjen M. Ruben Aachen Division of Nephrology and Transplant Medicine Department of Medicine Leiden University Medical Center Leiden The Netherlands Division of Nephrology and Transplant Medicine Department of Medicine, Leiden University Medical Center
Leiden University Medical Center · Cancer Center Amsterdam · Amsterdam UMC Location Vrije Universiteit Amsterdam
Affiliation as printed
Division of Nephrology and Transplant Medicine Department of Medicine Leiden University Medical Center Leiden The Netherlands
Division of Nephrology and Transplant Medicine, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
Present address: Amsterdam UMC, location VUmc, Cancer Center Amsterdam, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands
-
Leendert Adrianus Trouw Aachen Department of Immunology Leiden University Medical Center Leiden The Netherlands Department of Rheumatology Leiden University Medical Center Leiden The Netherlands Department of Immunology, Leiden University Medical Center Department of Rheumatology, Leiden University Medical Center
Leiden University Medical Center
Affiliation as printed
Department of Immunology Leiden University Medical Center Leiden The Netherlands
Department of Rheumatology Leiden University Medical Center Leiden The Netherlands
Department of Immunology, Leiden University Medical Center, Leiden, The Netherlands
Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands
-
Cees van Kooten corresponding Aachen Division of Nephrology and Transplant Medicine Department of Medicine Leiden University Medical Center Leiden The Netherlands Division of Nephrology and Transplant Medicine Department of Medicine, Leiden University Medical Center
Leiden University Medical Center
Affiliation as printed
Division of Nephrology and Transplant Medicine Department of Medicine Leiden University Medical Center Leiden The Netherlands
Division of Nephrology and Transplant Medicine, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
Cited by 8 stored of 8
8 results
No patents citing this paper on Lens.org (checked 2026-10-11).