Structure-based insights into evolution of rhodopsins
Communications Biology, vol. 4, pp. 821
Abstract
Rhodopsins, most of which are proton pumps generating transmembrane electrochemical proton gradients, span all three domains of life, are abundant in the biosphere, and could play a crucial role in the early evolution of life on earth. Whereas archaeal and bacterial proton pumps are among the best structurally characterized proteins, rhodopsins from unicellular eukaryotes have not been well characterized. To fill this gap in the current understanding of the proton pumps and to gain insight into the evolution of rhodopsins using a structure-based approach, we performed a structural and functional analysis of the light-driven proton pump LR (Mac) from the pathogenic fungus Leptosphaeria maculans. The first high-resolution structure of fungi rhodopsin and its functional properties reveal the striking similarity of its membrane part to archaeal but not to bacterial rhodopsins. We show that an unusually long N-terminal region stabilizes the protein through direct interaction with its extracellular loop (ECL2). We compare to our knowledge all available structures and sequences of outward light-driven proton pumps and show that eukaryotic and archaeal proton pumps, most likely, share a common ancestor.
Authors 21
-
Moscow Institute of Physics and Technology · Forschungszentrum Jülich
Affiliation as printed
Institute of Biological Information Processing (IBI-7: Structural Biochemistry), ForschungszentrumJülich, Jülich, Germany
JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich, Jülich, Germany
Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia
-
Affiliation as printed
Institute of Biological Information Processing (IBI-7: Structural Biochemistry), ForschungszentrumJülich, Jülich, Germany
JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich, Jülich, Germany
-
Moscow Institute of Physics and Technology · RWTH Aachen University
Affiliation as printed
Institute of Crystallography, University of Aachen (RWTH), Aachen, Germany
Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia
-
Centre National de la Recherche Scientifique · Moscow Institute of Physics and Technology · Commissariat à l'Énergie Atomique et aux Énergies Alternatives · Institut de Biologie Structurale · European Molecular Biology Laboratory · Université Grenoble Alpes
Affiliation as printed
European Molecular Biology Laboratory, Hamburg unit c/o DESY, Hamburg, Germany
Institut de Biologie Structurale (IBS), Université Grenoble Alpes-CEA-CNRS, Grenoble, France
Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia
-
Affiliation as printed
Institute of Biological Information Processing (IBI-7: Structural Biochemistry), ForschungszentrumJülich, Jülich, Germany
JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich, Jülich, Germany
-
Moscow Institute of Physics and Technology · Joint Institute for Nuclear Research · Institute for Safety Problems of Nuclear Power Plants
Affiliation as printed
Institute for Safety Problems of Nuclear Power Plants, NAS of Ukraine, Kyiv, Ukraine
Joint Institute for Nuclear Research, Dubna, Russia
Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia
-
Centre National de la Recherche Scientifique · Moscow Institute of Physics and Technology · Commissariat à l'Énergie Atomique et aux Énergies Alternatives · Institut de Biologie Structurale · Université Grenoble Alpes
Affiliation as printed
Institut de Biologie Structurale (IBS), Université Grenoble Alpes-CEA-CNRS, Grenoble, France
Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia
-
Centre National de la Recherche Scientifique · Moscow Institute of Physics and Technology · Forschungszentrum Jülich · Commissariat à l'Énergie Atomique et aux Énergies Alternatives · Institut de Biologie Structurale · RWTH Aachen University · Université Grenoble Alpes
Affiliation as printed
Institut de Biologie Structurale (IBS), Université Grenoble Alpes-CEA-CNRS, Grenoble, France
Institute of Biological Information Processing (IBI-7: Structural Biochemistry), ForschungszentrumJülich, Jülich, Germany
Institute of Crystallography, University of Aachen (RWTH), Aachen, Germany
JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich, Jülich, Germany
Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia
-
Moscow Institute of Physics and Technology · Forschungszentrum Jülich · RWTH Aachen University
Affiliation as printed
Institute of Biological Information Processing (IBI-7: Structural Biochemistry), ForschungszentrumJülich, Jülich, Germany
Institute of Crystallography, University of Aachen (RWTH), Aachen, Germany
JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich, Jülich, Germany
Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia
-
Forschungszentrum Jülich · European Molecular Biology Laboratory
Affiliation as printed
European Molecular Biology Laboratory, Hamburg Unit, Notkestrasse 25a, Hamburg, Germany
Institute of Biological Information Processing (IBI-7: Structural Biochemistry), ForschungszentrumJülich, Jülich, Germany
-
Centre National de la Recherche Scientifique · Forschungszentrum Jülich · Czech Academy of Sciences · Commissariat à l'Énergie Atomique et aux Énergies Alternatives · FZU - Institute of Physics of the Czech Academy of Sciences · Institut de Biologie Structurale · Université Grenoble Alpes
Affiliation as printed
ELI Beamlines, Institute of Physics, Czech Academy of Sciences, Prague, Czech Republic
Institut de Biologie Structurale (IBS), Université Grenoble Alpes-CEA-CNRS, Grenoble, France
Institute of Biological Information Processing (IBI-7: Structural Biochemistry), ForschungszentrumJülich, Jülich, Germany
-
Medizinische Hochschule Hannover
Affiliation as printed
Institute for Biophysical Chemistry, Hannover Medical School, Hannover, Germany
-
Moscow Institute of Physics and Technology · Joint Institute for Nuclear Research
Affiliation as printed
Joint Institute for Nuclear Research, Dubna, Russia
Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia
-
Moscow Institute of Physics and Technology
Affiliation as printed
Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia
-
Moscow Institute of Physics and Technology · Forschungszentrum Jülich
Affiliation as printed
Institute of Biological Information Processing (IBI-7: Structural Biochemistry), ForschungszentrumJülich, Jülich, Germany
JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich, Jülich, Germany
Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia
-
Moscow Institute of Physics and Technology
Affiliation as printed
Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia
-
Moscow Institute of Physics and Technology
Affiliation as printed
Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia
-
National Institutes of Health · National Center for Biotechnology Information
Affiliation as printed
National Center for Biotechnology Information, National Library of Medicine, National Institutesof Health, Bethesda, MD, United States
-
Max Planck Institute of Biophysics
Affiliation as printed
Max Planck Institute of Biophysics, Frankfurt am Main, Germany
-
National Institutes of Health · National Center for Biotechnology Information
Affiliation as printed
National Center for Biotechnology Information, National Library of Medicine, National Institutesof Health, Bethesda, MD, United States
-
Centre National de la Recherche Scientifique · Moscow Institute of Physics and Technology · Forschungszentrum Jülich · Commissariat à l'Énergie Atomique et aux Énergies Alternatives · Institut de Biologie Structurale · RWTH Aachen University · Université Grenoble Alpes
Affiliation as printed
Institut de Biologie Structurale (IBS), Université Grenoble Alpes-CEA-CNRS, Grenoble, France. valentin.gordeliy@ibs.fr
Institute of Biological Information Processing (IBI-7: Structural Biochemistry), ForschungszentrumJülich, Jülich, Germany. valentin.gordeliy@ibs.fr
Institute of Crystallography, University of Aachen (RWTH), Aachen, Germany. valentin.gordeliy@ibs.fr
JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich, Jülich, Germany. valentin.gordeliy@ibs.fr
Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia. valentin.gordeliy@ibs.fr
Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia
Cited by 34 stored of 34
No patents citing this paper on Lens.org (checked 2026-10-06).