Generation of T cell containing adipose tissue organoids

iScience, vol. 29, pp. 117429

Abstract

T cells and especially regulatory T cells play a pivotal role in adipose tissue homeostasis. Peripheral blood T cells are well studied; however, in-depth analysis of adipose tissue T cells in their environment is challenging, and the analysis of adipose tissue-resident T cells is currently restricted to in vivo mouse models and ex vivo human donor samples. We developed a model to study T cells in adipose tissue by means of an adipose tissue organoid model. We could observe a clear T cell infiltration in these organoids. Interestingly, we observed different infiltration ratios of regulatory T cells and CD4 + effector T cells. In addition to this, mimicking the inflammatory milieu in adipose tissue by the addition of lipopolysaccharide (LPS) enhanced CD4 + T cell infiltration within adipose tissue organoids. In summary, we herein provide a suitable ex vivo tool to analyze adipose tissue-resident T cells from different fat depots under near-physiological conditions.

Authors 12

1. C A Kühne Aachen Institute of Immunology, Medical Faculty Department of Pediatrics, Pediatric Rheumatology

   RWTH Aachen University · Georg Speyer Haus

   Affiliation as printed

   Department of Pediatrics, Pediatric Rheumatology, RWTH Aachen University, Aachen, Germany

   Institute for Tumor Biology and Experimental Therapy, Georg-Speyer-Haus, Frankfurt am Main, Germany

   Institute of Immunology, Medical Faculty, RWTH Aachen University, Pauwelstrasse 30, 52074 Aachen, Germany

2. Yasmin Majlesain

   University of Bonn

   Affiliation as printed

   Immunology and Environment, Life & Medical Sciences (LIMES) Institute, University of Bonn, Bonn, Germany

3. Sudheendra Hebbar Subramanyam Aachen Department of Pediatrics, Pediatric Rheumatology

   RWTH Aachen University

   Affiliation as printed

   Department of Pediatrics, Pediatric Rheumatology, RWTH Aachen University, Aachen, Germany

4. Christopher T. Neullens Aachen Department of Pediatrics, Pediatric Rheumatology

   RWTH Aachen University

   Affiliation as printed

   Department of Pediatrics, Pediatric Rheumatology, RWTH Aachen University, Aachen, Germany

5. Charlotte Lahrtz Aachen Institute of Immunology, Medical Faculty

   RWTH Aachen University

   Affiliation as printed

   Institute of Immunology, Medical Faculty, RWTH Aachen University, Pauwelstrasse 30, 52074 Aachen, Germany

6. Tim Ruhl Aachen Hand Surgery—Burn Center Department of Plastic Surgery

   RWTH Aachen University · Universitätsklinikum Aachen

   Affiliation as printed

   Department of Plastic Surgery, Hand Surgery-Burn Center, University Hospital RWTH Aachen, Aachen, Germany

7. Justus Beier Aachen Hand Surgery—Burn Center Department of Plastic Surgery

   RWTH Aachen University · Universitätsklinikum Aachen

   Affiliation as printed

   Department of Plastic Surgery, Hand Surgery-Burn Center, University Hospital RWTH Aachen, Aachen, Germany

8. Sabrina Bianca Bennstein Aachen Institute of Immunology, Medical Faculty

   RWTH Aachen University

   Affiliation as printed

   Institute of Immunology, Medical Faculty, RWTH Aachen University, Pauwelstrasse 30, 52074 Aachen, Germany

9. Lothar Rink Aachen Institute of Immunology, Medical Faculty

   RWTH Aachen University

   Affiliation as printed

   Institute of Immunology, Medical Faculty, RWTH Aachen University, Pauwelstrasse 30, 52074 Aachen, Germany

10. Klaus Tenbrock corresponding Aachen Department of Pediatrics, Pediatric Rheumatology

    RWTH Aachen University · University of Bern · University Hospital of Bern

    Affiliation as printed

    Department of Pediatrics, Pediatric Rheumatology, RWTH Aachen University, Aachen, Germany

    Division of Pediatric Rheumatology, Department of Pediatrics, Inselspital, University of Bern, Bern, Switzerland

11. Heike Weighardt corresponding

    University of Bonn

    Affiliation as printed

    Immunology and Environment, Life & Medical Sciences (LIMES) Institute, University of Bonn, Bonn, Germany

12. Kim Ohl corresponding Aachen Institute of Immunology, Medical Faculty Department of Pediatrics, Pediatric Rheumatology

    RWTH Aachen University · Hochschule Niederrhein

    Affiliation as printed

    Department of Pediatrics, Pediatric Rheumatology, RWTH Aachen University, Aachen, Germany

    Faculty of Food and Nutrition Sciences, University of Applied Sciences, Hochschule Niederrhein, Moenchengladbach, Germany

    Institute of Immunology, Medical Faculty, RWTH Aachen University, Pauwelstrasse 30, 52074 Aachen, Germany