Technical recommendations for analyzing oxylipins by liquid chromatography–mass spectrometry
Science Signaling, vol. 18, pp. eadw1245
Abstract
Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS.
Authors 93
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Nils Helge Schebb corresponding
Affiliation as printed
School of Mathematics and Natural Sciences, University of Wuppertal, Wuppertal, Germany
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Affiliation as printed
School of Mathematics and Natural Sciences, University of Wuppertal, Wuppertal, Germany
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Affiliation as printed
Unit of Integrative Metabolomics, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden
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Affiliation as printed
School of Mathematics and Natural Sciences, University of Wuppertal, Wuppertal, Germany
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Affiliation as printed
Applied Analytical Chemistry, University of Duisburg-Essen, Essen, Germany
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Affiliation as printed
Institute for Translational Medicine and Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA
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Affiliation as printed
Institute for Translational Medicine and Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA
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Instituto de Investigación Sanitaria La Fe
Affiliation as printed
Neonatal Research Group, Health Research Institute La Fe (IIS La Fe), Valencia, Spain
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Affiliation as printed
Department of Chemistry and Biochemistry, Concordia University, Montreal, Canada
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Karolinska Institutet · Victoria University of Wellington
Affiliation as printed
School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand
Unit of Integrative Metabolomics, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden
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Affiliation as printed
Department of Analytical Chemistry, University of Valencia, Burjassot, Spain
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Affiliation as printed
Mass Spectrometry Core Facility, Department of Chemistry, Columbia University, New York, NY, USA
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Georgia Institute of Technology
Affiliation as printed
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA
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University of California, Davis
Affiliation as printed
Department of Entomology, University of California, Davis, CA, USA
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Affiliation as printed
Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy, University of Greifswald, Greifswald, Germany
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Affiliation as printed
Unit of Integrative Metabolomics, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden
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University College Dublin · Queen's University Belfast
Affiliation as printed
Institute for Global Food Security, Queen’s University Belfast, Belfast, Northern Ireland
Nutrigenomics Research Group, UCD Conway Institute, School of Public Health, Physiotherapy and Sports Science, University College Dublin, Ireland
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University of California San Diego
Affiliation as printed
Department of Pharmacology and Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA, USA
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National Institutes of Health · National Institute of Environmental Health Sciences
Affiliation as printed
Division of Intramural Research, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, Durham, NC, USA
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National Institutes of Health · National Institute of Environmental Health Sciences
Affiliation as printed
Division of Intramural Research, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, Durham, NC, USA
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Affiliation as printed
BPG Bio, Framingham, MA, USA
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Affiliation as printed
Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil
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Affiliation as printed
Nestlé Institute of Food Safety and Analytical Sciences, Société des Produits Nestlé, Lausanne, Switzerland
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Hebrew University of Jerusalem
Affiliation as printed
Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel
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Affiliation as printed
Lipidomix GmbH, Berlin, Germany
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Affiliation as printed
Shimadzu UK Ltd., Milton Keynes, UK
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Affiliation as printed
Institute for Translational Medicine and Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA
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Affiliation as printed
School of Mathematics and Natural Sciences, University of Wuppertal, Wuppertal, Germany
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University of California, Davis
Affiliation as printed
Department of Food Science and Technology, University of California, Davis, CA, USA
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Friedrich Schiller University Jena
Affiliation as printed
Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich Schiller University, Jena, Germany
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Friedrich Schiller University Jena
Affiliation as printed
Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich Schiller University, Jena, Germany
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Friedrich Schiller University Jena
Affiliation as printed
Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich Schiller University, Jena, Germany
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Affiliation as printed
Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany
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Université Toulouse III - Paul Sabatier · Inserm · MetaboHUB · Institut des Maladies Métaboliques et Cardiovasculaires · Université de Toulouse
Affiliation as printed
MetaboHUB-MetaToul, I2MC, Université de Toulouse, Inserm, Université Toulouse III–Paul Sabatier (UPS), Toulouse, France
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Université Toulouse III - Paul Sabatier · Inserm · MetaboHUB · Institut des Maladies Métaboliques et Cardiovasculaires · Université de Toulouse
Affiliation as printed
MetaboHUB-MetaToul, I2MC, Université de Toulouse, Inserm, Université Toulouse III–Paul Sabatier (UPS), Toulouse, France
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Goethe University Frankfurt · Fraunhofer Institute for Translational Medicine and Pharmacology
Affiliation as printed
Faculty of Medicine, Institute of Clinical Pharmacology, Goethe University Frankfurt, Frankfurt am Main, Germany
Fraunhofer Cluster of Excellence of Immune Mediate Diseases CIMD, Frankfurt am Main, Germany
Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Frankfurt am Main, Germany
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Goethe University Frankfurt · Fraunhofer Institute for Translational Medicine and Pharmacology
Affiliation as printed
Faculty of Medicine, Institute of Clinical Pharmacology, Goethe University Frankfurt, Frankfurt am Main, Germany
Fraunhofer Cluster of Excellence of Immune Mediate Diseases CIMD, Frankfurt am Main, Germany
Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Frankfurt am Main, Germany
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National University of Singapore · Duke-NUS Medical School · Life Sciences Institute
Affiliation as printed
Signature Research Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore (NUS) Medical School, Singapore, Singapore
Singapore Lipidomics Incubator, Life Sciences Institute and Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
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Affiliation as printed
School of Biosciences & Aston Institute for Membrane Excellence, Aston University, Aston Triangle, Birmingham, UK
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Affiliation as printed
Aston Medical School, College of Health and Life Sciences, Aston University, Aston Triangle, Birmingham, UK
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Affiliation as printed
School of Biosciences & Aston Institute for Membrane Excellence, Aston University, Aston Triangle, Birmingham, UK
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Affiliation as printed
Department of Chemistry and Industrial Chemistry, University of Pisa, Pisa, Italy
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Affiliation as printed
Department of Chemistry and Industrial Chemistry, University of Pisa, Pisa, Italy
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Karolinska University Hospital · Karolinska Institutet
Affiliation as printed
Division of Rheumatology, Department of Medicine Solna, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden
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Affiliation as printed
Center for Novel Target & Therapeutic Intervention, College of Pharmacy, Chongqing Medical University, Chongqing, P. R. China
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University Hospital Carl Gustav Carus · Technische Universität Dresden
Affiliation as printed
Center of Membrane Biochemistry and Lipid Research, University Hospital and Faculty of Medicine Carl Gustav Carus of TU Dresden, Dresden, Germany
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Affiliation as printed
Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University, St. Louis, MO, USA
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The University of Western Australia · Royal Perth Hospital
Affiliation as printed
Medical School, Royal Perth Hospital Unit, University of Western Australia, Perth, Australia
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The University of Western Australia · Royal Perth Hospital
Affiliation as printed
Medical School, Royal Perth Hospital Unit, University of Western Australia, Perth, Australia
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Cayman Chemical (United States)
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Cayman Chemical Company, Ann Arbor, MI, USA
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Cayman Chemical (United States)
Affiliation as printed
Cayman Chemical Company, Ann Arbor, MI, USA
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Affiliation as printed
Metabolomics and Lipidomics Platform, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland
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Affiliation as printed
Metabolomics and Lipidomics Platform, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland
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Université Clermont Auvergne · Unité de Nutrition Humaine · Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement · Centre de Recherche en Nutrition Humaine d'Auvergne
Affiliation as printed
Université Clermont Auvergne, INRAE, UNH, Unité de Nutrition Humaine, CRNH Auvergne, Clermont-Ferrand, France
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National University of Singapore · Hamad bin Khalifa University
Affiliation as printed
College of Health and Life Sciences, Hamad Bin Khalifa University, Doha, Qatar
Long Loo Lin School of Medicine, National University of Singapore
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Centre National de la Recherche Scientifique · Université de Montpellier · Institut des Biomolécules Max Mousseron
Affiliation as printed
Institut des Biomolécules Max Mousseron (IBMM), Pôle Chimie Balard Recherche, CNRS, Université de Montpellier, ENSCN, Montpellier, France
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Centre National de la Recherche Scientifique · Université de Montpellier · Institut des Biomolécules Max Mousseron
Affiliation as printed
Institut des Biomolécules Max Mousseron (IBMM), Pôle Chimie Balard Recherche, CNRS, Université de Montpellier, ENSCN, Montpellier, France
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Affiliation as printed
Department of Kinesiology and Health Sciences, University of Waterloo, Waterloo, Canada
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Affiliation as printed
Centre for Metabolomics and Bioanalysis (CEMBIO), Faculty of Pharmacy, CEU San Pablo University, Madrid, Spain
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Affiliation as printed
Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy, University of Greifswald, Greifswald, Germany
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University Hospital Leipzig · Leipzig University
Affiliation as printed
Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University of Leipzig Medical Center, Leipzig, Germany
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Institut universitaire de cardiologie et de pneumologie de Québec · Université Laval
Affiliation as printed
Centre de recherche de l’Institut universitaire de cardiologie et de pneumologie de Québec, Département de médecine, Faculté de médecine, Université Laval, Québec City, Canada
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Affiliation as printed
Rowett Institute, School of Medicine, Medical Science and Nutrition, University of Aberdeen, Aberdeen, UK
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Affiliation as printed
Department of Analytical Chemistry, University of Vienna, Vienna, Austria
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RIKEN Center for Integrative Medical Sciences
Affiliation as printed
RIKEN Center for Integrative Medical Sciences (IMS), Yokohama, Kanagawa, Japan
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National Institutes of Health · National Institute of Environmental Health Sciences
Affiliation as printed
Division of Intramural Research, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, Durham, NC, USA
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Affiliation as printed
Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, USA
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University of California San Diego
Affiliation as printed
Department of Pharmacology and Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA, USA
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Indigo BioAutomation, Carmel, IN, USA
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Affiliation as printed
Division of Pharmacy and Optometry, School of Health Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK
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Affiliation as printed
Department of Systems Pharmacology and Translational Therapeutics, University of Pennsylvania, Philadelphia, PA, USA
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University of Cambridge · MRC Mitochondrial Biology Unit · Medical Research Council
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Medical Research Council Mitochondrial Biology Unit and Department of Medicine, University of Cambridge, Cambridge, UK
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University of California, Davis
Affiliation as printed
Department of Entomology, University of California, Davis, CA, USA
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Affiliation as printed
Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, USA
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University Hospital Regensburg
Affiliation as printed
Institute of Clinical Chemistry and Laboratory Medicine, University Hospital Regensburg, Regensburg, Germany
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Brigham and Women's Hospital · Harvard University
Affiliation as printed
Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Hale Building for Transformative Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, USA
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Affiliation as printed
Core Facility Mass Spectrometry, ZMF, Medical University of Graz, Graz, Austria
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Karolinska University Hospital · Karolinska Institutet
Affiliation as printed
Division of Rheumatology, Department of Medicine Solna, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden
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Affiliation as printed
Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany
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Affiliation as printed
Department of Chemistry, University of Washington, Seattle, WA, USA
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Affiliation as printed
Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic
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The Queen's Medical Research Institute · University of Edinburgh
Affiliation as printed
Centre for Cardiovascular Science, Queen’s Medical Research Institute, University of Edinburgh, Edinburgh, UK
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Martin A. Giera Aachen
Leiden University Medical Center
Affiliation as printed
Leiden University Medical Center, Leiden, Netherlands
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Affiliation as printed
Institute for Translational Medicine and Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA
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Affiliation as printed
Department of Pharmacology, University of Colorado Denver, Denver, CO, USA
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Western Human Nutrition Research Center · University of California, Davis
Affiliation as printed
USDA ARS Western Human Nutrition Research Center and Department of Nutrition, University of California, Davis, CA, USA
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University of California San Diego
Affiliation as printed
Department of Pharmacology and Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA, USA
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Affiliation as printed
Lipidomics Consulting Ltd., Esbo, Finland
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Vanderbilt University · Vanderbilt University Medical Center
Affiliation as printed
Division of Clinical Pharmacology, Department of Medicine, Vanderbilt University Medical Centre, Nashville, TN, USA
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Cayman Chemical (United States)
Affiliation as printed
Cayman Chemical Company, Ann Arbor, MI, USA
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Centers for Disease Control and Prevention
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Centers for Disease Control and Prevention, Atlanta, GA, USA
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Karolinska University Hospital · Karolinska Institutet
Affiliation as printed
Department of Respiratory Medicine and Allergy, Karolinska University Hospital, Stockholm, Sweden
Unit of Integrative Metabolomics, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden
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Affiliation as printed
School of Medicine, Cardiff University, Heath Park, Cardiff, UK
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