The IBEX Knowledge-Base: A central resource for multiplexed imaging techniques

PLoS Biology, vol. 23, pp. e3003070

Abstract

Multiplexed imaging is a powerful approach in spatial biology, although it is complex, expensive and labor-intensive. Here, we present the IBEX Knowledge-Base, a central resource for reagents, protocols and more, to enhance knowledge sharing, optimization and innovation of spatial proteomics techniques.

Authors 62

1. Andrea J. Radtke corresponding

   National Institutes of Health · National Institute of Allergy and Infectious Diseases

   Affiliation as printed

   Lymphocyte Biology Section and Center for Advanced Tissue Imaging, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

2. Ifeanyichukwu U. Anidi

   National Institutes of Health · National Heart, Lung, and Blood Institute

   Affiliation as printed

   Critical Care Medicine and Pulmonary Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America

3. Leanne Arakkal

   National Institutes of Health · National Institute of Allergy and Infectious Diseases

   Affiliation as printed

   Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

4. Armando Arroyo-Mejías

   National Institutes of Health · National Institute of Allergy and Infectious Diseases

   Affiliation as printed

   Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

5. Rebecca T. Beuschel

   National Institutes of Health · National Institute of Allergy and Infectious Diseases

   Affiliation as printed

   Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

6. Katy Börner

   Indiana University Bloomington · Indiana University

   Affiliation as printed

   Department of Intelligent Systems Engineering, Indiana University, Bloomington, Indiana, United States of America

7. Colin J. Chu

   NIHR Moorfields Biomedical Research Centre · University College London

   Affiliation as printed

   UCL Institute of Ophthalmology and NIHR Moorfields Biomedical Research Centre, London, United Kingdom

8. Beatrice Clark

   National Institutes of Health · National Institute of Allergy and Infectious Diseases

   Affiliation as printed

   Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

9. Menna Ruth Clatworthy

   University of Cambridge · Molecular Immunity Unit

   Affiliation as printed

   Molecular Immunity Unit, Laboratory of Molecular Biology, Cambridge Institute for Therapeutic Immunology and Infectious Diseases, University of Cambridge Department of Medicine, Cambridge, United Kingdom

10. Jake Colautti

    McMaster University

    Affiliation as printed

    Department of Medicine, McMaster Immunology Research Centre, Schroeder Allergy and Immunology Research Institute, Faculty of Health Sciences, McMaster University, Hamilton, Canada

11. Fabian Coscia

    Max Delbrück Center

    Affiliation as printed

    Max-Delbrueck-Center for Molecular Medicine in the Helmholtz Association (MDC), Spatial Proteomics Group, Berlin, Germany

12. Joshua D. Croteau

    BioLegend (United States)

    Affiliation as printed

    Department of Business Development, BioLegend Inc., San Diego, California, United States of America

13. Saven Denha

    McMaster University

    Affiliation as printed

    Department of Medicine, McMaster Immunology Research Centre, Schroeder Allergy and Immunology Research Institute, Faculty of Health Sciences, McMaster University, Hamilton, Canada

14. Rose Dever

    National Institutes of Health · National Institute of Arthritis and Musculoskeletal and Skin Diseases

    Affiliation as printed

    Functional Immunogenomics Unit, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

15. Walderez O. Dutra

    Universidade Federal de Minas Gerais

    Affiliation as printed

    Laboratory of Cell-Cell Interactions, Department of Morphology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

16. Sonja Fritzsche

    Max Delbrück Center

    Affiliation as printed

    Max-Delbrueck-Center for Molecular Medicine in the Helmholtz Association (MDC), Spatial Proteomics Group, Berlin, Germany

17. Spencer Fullam

    Rush University Medical Center

    Affiliation as printed

    Division of Rheumatology, Rush University Medical Center, Chicago, Illinois, United States of America

18. Michael Y. Gerner

    University of Washington

    Affiliation as printed

    Department of Immunology, University of Washington School of Medicine, Seattle, Washington, United States of America

19. Anita Gola

    Rockefeller University

    Affiliation as printed

    Robin Chemers Neustein Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, New York, United States of America

20. Kenneth J. Gollob

    Hospital Israelita Albert Einstein

    Affiliation as printed

    Center for Research in Immuno-oncology (CRIO), Hospital Israelita Albert Einstein, São Paulo, Brazil

21. Jonathan Matthew Hernandez

    National Institutes of Health · National Cancer Institute

    Affiliation as printed

    Surgical Oncology Program, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America

22. Jyh Liang Hor

    National Institutes of Health · National Institute of Allergy and Infectious Diseases

    Affiliation as printed

    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

23. Hiroshi Ichise

    National Institutes of Health · National Institute of Allergy and Infectious Diseases

    Affiliation as printed

    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

24. Zhixin Jing

    National Institutes of Health · National Institute of Allergy and Infectious Diseases

    Affiliation as printed

    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

25. Danny David Jonigk Aachen Institute of Pathology, Aachen Medical University

    German Center for Lung Research · RWTH Aachen University

    Affiliation as printed

    German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany

    Institute of Pathology, Aachen Medical University, RWTH Aachen, Aachen, Germany

26. Evelyn Kandov

    National Institutes of Health · National Institute of Allergy and Infectious Diseases

    Affiliation as printed

    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

27. Wolfgang Kastenmüller

    Max Planck Society · University of Würzburg

    Affiliation as printed

    Würzburg Institute of Systems Immunology, Max Planck Research Group at the Julius-Maximilians-Universität Würzburg, Würzburg, Germany

28. Joshua F. E. Koenig

    McMaster University

    Affiliation as printed

    Department of Medicine, McMaster Immunology Research Centre, Schroeder Allergy and Immunology Research Institute, Faculty of Health Sciences, McMaster University, Hamilton, Canada

29. Rosa K. Kortekaas

    St. Joseph’s Healthcare Hamilton · McMaster University

    Affiliation as printed

    Department of Medicine, McMaster University, Firestone Institute for Respiratory Health, St Joseph's Healthcare, Hamilton, Canada

30. Aanandita A. Kothurkar

    NIHR Moorfields Biomedical Research Centre · University College London

    Affiliation as printed

    UCL Institute of Ophthalmology and NIHR Moorfields Biomedical Research Centre, London, United Kingdom

31. Alexandra Y. Kreins

    Great Ormond Street Hospital · Great Ormond Street Hospital for Children NHS Foundation Trust · University College London

    Affiliation as printed

    Department of Immunology and Gene Therapy, Great Ormond Street Hospital for Children NHS Foundation Trust, London, United Kingdom

    Infection Immunity and Inflammation Research and Teaching Department, University College London Great Ormond Street Institute of Child Health, London, United Kingdom

32. Ian T. Lamborn

    National Institutes of Health · National Institute of Allergy and Infectious Diseases

    Affiliation as printed

    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

33. Yuri Lin

    National Institutes of Health · National Cancer Institute

    Affiliation as printed

    Surgical Oncology Program, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America

34. Kátia L.P. Morais

    Hospital Israelita Albert Einstein

    Affiliation as printed

    Center for Research in Immuno-oncology (CRIO), Hospital Israelita Albert Einstein, São Paulo, Brazil

35. Aleksandra Lunich

    National Institutes of Health · National Heart, Lung, and Blood Institute

    Affiliation as printed

    Critical Care Medicine and Pulmonary Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America

36. Jean CS Luz

    Universidade de São Paulo

    Affiliation as printed

    Viral Vector Laboratory, Cancer Institute of São Paulo, University of São Paulo, São Paulo, Brazil

37. Ryan B. MacDonald

    NIHR Moorfields Biomedical Research Centre · University College London

    Affiliation as printed

    UCL Institute of Ophthalmology and NIHR Moorfields Biomedical Research Centre, London, United Kingdom

38. Chen Makranz

    National Institutes of Health · National Cancer Institute

    Affiliation as printed

    Neuro-Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America

39. Vivien I. Maltez

    University of California San Diego

    Affiliation as printed

    Division of Allergy, Immunology and Rheumatology, Department of Pediatrics, University of California San Diego, La Jolla, California, United States of America

40. John E. McDonough

    St. Joseph’s Healthcare Hamilton · McMaster University

    Affiliation as printed

    Department of Medicine, McMaster University, Firestone Institute for Respiratory Health, St Joseph's Healthcare, Hamilton, Canada

41. Ryan V. Moriarty

    Northwestern University

    Affiliation as printed

    Department of Cellular and Developmental Biology, Northwestern University, Chicago, Illinois, United States of America

42. Juan Moisés Ocampo‐Godínez

    University College London · Instituto Politécnico Nacional · Universidad Nacional Autónoma de México

    Affiliation as printed

    Infection Immunity and Inflammation Research and Teaching Department, University College London Great Ormond Street Institute of Child Health, London, United Kingdom

    Laboratorio de Bioingeniería de Tejidos, Departamento de Estudios de Posgrado e Investigación, Universidad Nacional Autónoma de México, Mexico City, Mexico

    Laboratorio de Inmunoquímica I, Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico

43. Vitoria M Olyntho

    McMaster University

    Affiliation as printed

    Department of Medicine, McMaster Immunology Research Centre, Schroeder Allergy and Immunology Research Institute, Faculty of Health Sciences, McMaster University, Hamilton, Canada

44. Annette Oxenius

    ETH Zurich · Institute of Microbiology

    Affiliation as printed

    Institute of Microbiology, ETH Zurich, Zurich, Switzerland

45. Kartika Padhan

    National Institutes of Health · National Institute of Allergy and Infectious Diseases

    Affiliation as printed

    Lymphocyte Biology Section and Center for Advanced Tissue Imaging, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

46. Kirsten Remmert

    National Institutes of Health · National Cancer Institute

    Affiliation as printed

    Surgical Oncology Program, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America

47. Nathan Richoz

    University of Cambridge · Molecular Immunity Unit

    Affiliation as printed

    Molecular Immunity Unit, Laboratory of Molecular Biology, Cambridge Institute for Therapeutic Immunology and Infectious Diseases, University of Cambridge Department of Medicine, Cambridge, United Kingdom

48. Edward C. Schrom

    National Institutes of Health · National Institute of Allergy and Infectious Diseases

    Affiliation as printed

    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

49. Wanjing Shang

    National Institutes of Health · National Institute of Allergy and Infectious Diseases

    Affiliation as printed

    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

50. Lihong Shi

    National Institutes of Health · National Institute of Allergy and Infectious Diseases

    Affiliation as printed

    Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

51. Rochelle M. Shih

    National Institutes of Health · National Institute of Allergy and Infectious Diseases

    Affiliation as printed

    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

52. Emily E. Speranza

    Cleveland Clinic

    Affiliation as printed

    Florida Research and Innovation Center, Cleveland Clinic Lerner Research Institute, Port Saint Lucie, Florida, United States of America

53. Salome Stierli

    University of Zurich

    Affiliation as printed

    Institute of Anatomy, University of Zurich, Zurich, Switzerland

54. Sarah Amalia Teichmann

    Wellcome/MRC Cambridge Stem Cell Institute · University of Cambridge

    Affiliation as printed

    Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, Puddicombe Way, Cambridge Biomedical Campus, Cambridge, United Kingdom

    Department of Medicine, University of Cambridge, Cambridge, United Kingdom

55. Tibor Z. Veres

    National Institutes of Health · National Institute of Allergy and Infectious Diseases

    Affiliation as printed

    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

56. Megan Vierhout

    McMaster University

    Affiliation as printed

    Department of Medicine, McMaster Immunology Research Centre, Schroeder Allergy and Immunology Research Institute, Faculty of Health Sciences, McMaster University, Hamilton, Canada

57. Brianna T. Wachter

    National Institutes of Health · National Institute of Allergy and Infectious Diseases

    Affiliation as printed

    Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

58. Adam K. Wade‐Vallance

    National Institutes of Health · National Institute of Allergy and Infectious Diseases

    Affiliation as printed

    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

59. Margaret C. Williams

    National Institutes of Health · National Heart, Lung, and Blood Institute

    Affiliation as printed

    Critical Care Medicine and Pulmonary Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America

60. Nathan Sébastien Zangger

    ETH Zurich · Institute of Microbiology

    Affiliation as printed

    Institute of Microbiology, ETH Zurich, Zurich, Switzerland

61. Ronald N. Germain corresponding

    National Institutes of Health · National Institute of Allergy and Infectious Diseases

    Affiliation as printed

    Lymphocyte Biology Section and Center for Advanced Tissue Imaging, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

62. Ziv Rafael Yaniv corresponding

    National Institutes of Health · National Institute of Allergy and Infectious Diseases

    Affiliation as printed

    Bioinformatics and Computational Bioscience Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America