Label-free single-cell RNA multiplexing leveraging genetic variability

Nature Communications, vol. 15, pp. 10612

Abstract

Single cell RNA sequencing has provided unprecedented insights into the molecular cues and cellular heterogeneity underlying human disease. However, the high costs and complexity of single cell methods remain a major obstacle for generating large-scale human cohorts. Here, we compare current state-of-the-art single cell multiplexing technologies, and provide a widely applicable demultiplexing method, SoupLadle, that enables simple, yet robust high-throughput multiplexing leveraging genetic variability of patients.

Authors 13

1. Konrad Hoeft Aachen Department of Medicine 2 (Nephrology Clinical Immunology and Hypertension) Medical Faculty

   Erasmus MC · RWTH Aachen University

   Affiliation as printed

   Department of Internal Medicine, Nephrology and Transplantation, Erasmus Medical Center, Rotterdam, The Netherlands

   Department of Medicine 2 (Nephrology, Rheumatology, Clinical Immunology and Hypertension), Medical Faculty, RWTH Aachen University, Aachen, Germany

2. Tore Bleckwehl Aachen Department of Medicine 2 (Nephrology Clinical Immunology and Hypertension) Medical Faculty

   RWTH Aachen University

   Affiliation as printed

   Department of Medicine 2 (Nephrology, Rheumatology, Clinical Immunology and Hypertension), Medical Faculty, RWTH Aachen University, Aachen, Germany

3. David Schumacher Aachen Department of Anesthesiology, Medical Faculty Department of Medicine 2 (Nephrology Clinical Immunology and Hypertension) Medical Faculty

   RWTH Aachen University

   Affiliation as printed

   Department of Anesthesiology, Medical Faculty, RWTH Aachen University, Aachen, Germany

   Department of Medicine 2 (Nephrology, Rheumatology, Clinical Immunology and Hypertension), Medical Faculty, RWTH Aachen University, Aachen, Germany

4. Hyojin Kim Aachen Department of Medicine 2 (Nephrology Clinical Immunology and Hypertension) Medical Faculty

   RWTH Aachen University

   Affiliation as printed

   Department of Medicine 2 (Nephrology, Rheumatology, Clinical Immunology and Hypertension), Medical Faculty, RWTH Aachen University, Aachen, Germany

5. Robert Meyer Aachen Institute for Human Genetics and Genomic Medicine Medical Faculty

   RWTH Aachen University

   Affiliation as printed

   Institute for Human Genetics and Genomic Medicine, Medical Faculty, RWTH Aachen University, Aachen, Germany

6. Ling Zhang Aachen Department of Medicine 2 (Nephrology Clinical Immunology and Hypertension) Medical Faculty

   RWTH Aachen University

   Affiliation as printed

   Department of Medicine 2 (Nephrology, Rheumatology, Clinical Immunology and Hypertension), Medical Faculty, RWTH Aachen University, Aachen, Germany

7. Marian C. Clahsen‐van Groningen Aachen Department of Medicine 2 (Nephrology Clinical Immunology and Hypertension) Medical Faculty

   Erasmus MC · RWTH Aachen University

   Affiliation as printed

   Department of Medicine 2 (Nephrology, Rheumatology, Clinical Immunology and Hypertension), Medical Faculty, RWTH Aachen University, Aachen, Germany

   Department of Pathology and Clinical Bioinformatics, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands

8. Anne Babler Aachen Department of Medicine 2 (Nephrology Clinical Immunology and Hypertension) Medical Faculty

   RWTH Aachen University

   Affiliation as printed

   Department of Medicine 2 (Nephrology, Rheumatology, Clinical Immunology and Hypertension), Medical Faculty, RWTH Aachen University, Aachen, Germany

9. Turgay Saritas Aachen Department of Medicine 2 (Nephrology Clinical Immunology and Hypertension) Medical Faculty

   Erasmus MC · RWTH Aachen University

   Affiliation as printed

   Department of Internal Medicine, Nephrology and Transplantation, Erasmus Medical Center, Rotterdam, The Netherlands

   Department of Medicine 2 (Nephrology, Rheumatology, Clinical Immunology and Hypertension), Medical Faculty, RWTH Aachen University, Aachen, Germany

10. Ingo Kurth Aachen Institute for Human Genetics and Genomic Medicine Medical Faculty

    RWTH Aachen University

    Affiliation as printed

    Institute for Human Genetics and Genomic Medicine, Medical Faculty, RWTH Aachen University, Aachen, Germany

11. Hendrik Milting

    Heart and Diabetes Center North Rhine-Westphalia

    Affiliation as printed

    Erich and Hanna Klessmann Institute for Cardiovascular Research and Development, Clinic for Thoracic and Cardiovascular Surgery, Heart and Diabetes Center NRW, Bad Oeynhausen, Germany

12. Sikander Hayat corresponding Aachen Department of Medicine 2 (Nephrology Clinical Immunology and Hypertension) Medical Faculty

    RWTH Aachen University

    Affiliation as printed

    Department of Medicine 2 (Nephrology, Rheumatology, Clinical Immunology and Hypertension), Medical Faculty, RWTH Aachen University, Aachen, Germany. shayat@ukaachen.de

    Department of Medicine 2 (Nephrology, Rheumatology, Clinical Immunology and Hypertension), Medical Faculty, RWTH Aachen University, Aachen, Germany

13. Rafael Kramann corresponding Aachen Department of Medicine 2 (Nephrology Clinical Immunology and Hypertension) Medical Faculty

    Erasmus MC · RWTH Aachen University

    Affiliation as printed

    Department of Internal Medicine, Nephrology and Transplantation, Erasmus Medical Center, Rotterdam, The Netherlands. rkramann@ukaachen.de

    Department of Medicine 2 (Nephrology, Rheumatology, Clinical Immunology and Hypertension), Medical Faculty, RWTH Aachen University, Aachen, Germany. rkramann@ukaachen.de

    Department of Internal Medicine, Nephrology and Transplantation, Erasmus Medical Center, Rotterdam, The Netherlands