Enhanced cell wall mechanics in VirtualLeaf enable realistic simulations of plant tissue dynamics

bioRxiv (Cold Spring Harbor Laboratory)

Abstract

Abstract Computational modelling has become essential to advancing our understanding of plant developmental and physiological processes, necessitating the development of new computational approaches and software. Here, we present VirtualLeaf-2.0, an updated version of this modelling framework for the biophysical and biomechanical interactions between cells in plant tissues, with novel features for more detailed modelling of the cell wall. In particular, the updated version of VirtualLeaf enables detailed modelling of variations in cell wall stability and cell wall sliding up to the level of individual cell wall elements. The plant cell wall plays a pivotal role in plant development and survival, with younger cells generally having thinner, more flexible (primary) walls than older cells. Cell wall stability is further affected by signalling in growth processes and pathogen infection. The improvements of VirtualLeaf lay the groundwork for using VirtualLeaf to address novel questions involving plant tissue dynamics during growth, tissue formation and pathogen defence, as illustrated with example simulations.

Authors 4

1. Richard W. van Nieuwenhoven corresponding

   TU Wien · Heidelberg University · Maastricht University

   Affiliation as printed

   Institute of Applied Physics, TU Wien

   BioQuant, Heidelberg University, Heidelberg, Germany

   Brightlands Future Farming Insitute, Maastricht University, Venlo, Netherlands

   Maastricht Centre for Systems Biology, Maastricht University, Maastricht, Netherlands

2. Bruno Hay Mele

   TU Wien · University of Naples Federico II

   Affiliation as printed

   Department of Biology, University of Naples

   Institute of Applied Physics, TU Wien, Vienna, Austria

3. Roeland M. H. Merks Aachen Institute for Biology Leiden Mathematical Institute

   Leiden University · University of Naples Federico II

   Affiliation as printed

   Institute for Biology Leiden (IBL), Leiden University

   Mathematical Institute, Leiden University

   Department of Biology, University of Naples, Naples, Italy

4. Ruth Großeholz Aachen Institute for Biology Leiden Mathematical Institute

   Leiden University · Heidelberg University · Maastricht University

   Affiliation as printed

   BioQuant, Heidelberg University

   Brightlands Future Farming Insitute, Maastricht University

   Maastricht Centre for Systems Biology, Maastricht University

   Institute for Biology Leiden (IBL), Leiden University, Leiden, Netherlands

   Mathematical Institute, Leiden University, Leiden, Netherlands