Exploring engineered vesiculation by Pseudomonas putida KT2440 for natural product biosynthesis
Microbial Biotechnology, vol. 17, pp. e14312
Abstract
Pseudomonas species have become promising cell factories for the production of natural products due to their inherent robustness. Although these bacteria have naturally evolved strategies to cope with different kinds of stress, many biotechnological applications benefit from engineering of optimised chassis strains with specially adapted tolerance traits. Here, we explored the formation of outer membrane vesicles (OMV) of Pseudomonas putida KT2440. We found OMV production to correlate with the recombinant production of a natural compound with versatile beneficial properties, the tripyrrole prodigiosin. Further, several P. putida genes were identified, whose up- or down-regulated expression allowed controlling OMV formation. Finally, genetically triggering vesiculation in production strains of the different alkaloids prodigiosin, violacein, and phenazine-1-carboxylic acid, as well as the carotenoid zeaxanthin, resulted in up to three-fold increased product yields. Consequently, our findings suggest that the construction of robust strains by genetic manipulation of OMV formation might be developed into a useful tool which may contribute to improving limited biotechnological applications.
Authors 16
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Heinrich Heine University Düsseldorf
Affiliation as printed
Institute of Molecular Enzyme Technology (IMET) Heinrich Heine University Düsseldorf Düsseldorf Germany
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Heinrich Heine University Düsseldorf
Affiliation as printed
Institute of Molecular Enzyme Technology (IMET) Heinrich Heine University Düsseldorf Düsseldorf Germany
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Heinrich Heine University Düsseldorf
Affiliation as printed
Institute of Molecular Enzyme Technology (IMET) Heinrich Heine University Düsseldorf Düsseldorf Germany
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Heinrich Heine University Düsseldorf
Affiliation as printed
Institute of Molecular Enzyme Technology (IMET) Heinrich Heine University Düsseldorf Düsseldorf Germany
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Helmholtz Centre for Environmental Research
Affiliation as printed
Department of Environmental Biotechnology Helmholtz Centre for Environmental Research (UFZ) Leipzig Germany
Department of Environmental Biotechnology, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany
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Helmholtz Centre for Environmental Research
Affiliation as printed
Department of Environmental Biotechnology Helmholtz Centre for Environmental Research (UFZ) Leipzig Germany
Department of Environmental Biotechnology, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany
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Affiliation as printed
Institute of Biological Information Processing – Structural Biochemistry (IBI‐7: Structural Biochemistry) Forschungszentrum Jülich Jülich Germany
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Forschungszentrum Jülich · Ernst Ruska Centre
Affiliation as printed
Ernst‐Ruska Centre for Microscopy and Spectroscopy with Electrons (ER‐C‐3/Structural Biology) Forschungszentrum Jülich Jülich Germany
Institute for Biological Information Processing 6 (IBI‐6/ Structural Cellular Biology) Forschungszentrum Jülich Jülich Germany
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Forschungszentrum Jülich · Ernst Ruska Centre
Affiliation as printed
Ernst‐Ruska Centre for Microscopy and Spectroscopy with Electrons (ER‐C‐3/Structural Biology) Forschungszentrum Jülich Jülich Germany
Institute for Biological Information Processing 6 (IBI‐6/ Structural Cellular Biology) Forschungszentrum Jülich Jülich Germany
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Forschungszentrum Jülich · Ernst Ruska Centre · Heinrich Heine University Düsseldorf
Affiliation as printed
Department of Biology Heinrich Heine University Düsseldorf Düsseldorf Germany
Ernst‐Ruska Centre for Microscopy and Spectroscopy with Electrons (ER‐C‐3/Structural Biology) Forschungszentrum Jülich Jülich Germany
Institute for Biological Information Processing 6 (IBI‐6/ Structural Cellular Biology) Forschungszentrum Jülich Jülich Germany
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Affiliation as printed
Bielefeld University, Medical School East Westphalia‐Lippe Bielefeld University Bielefeld Germany
Center for Biotechnology (CeBiTec) Bielefeld University Bielefeld Germany
Bielefeld University, Medical School East Westphalia-Lippe, Bielefeld University, Bielefeld, Germany
Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany
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Affiliation as printed
Center for Biotechnology (CeBiTec) Bielefeld University Bielefeld Germany
Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany
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Thomke Belthle Aachen DWI – Leibniz Institute for Interactive Materials Aachen Germany Functional and Interactive Polymers Institute of Technical and Macromolecular Chemistry
DWI – Leibniz Institute for Interactive Materials · RWTH Aachen University
Affiliation as printed
DWI─Leibniz‐Institute for Interactive Materials Aachen Germany
Functional and Interactive Polymers, Institute of Technical and Macromolecular Chemistry RWTH Aachen University Aachen Germany
Functional and Interactive Polymers, Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Aachen, Germany
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Heinrich Heine University Düsseldorf
Affiliation as printed
Institute of Molecular Enzyme Technology (IMET) Heinrich Heine University Düsseldorf Düsseldorf Germany
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Forschungszentrum Jülich · Heinrich Heine University Düsseldorf
Affiliation as printed
Institute of Bio‐ and Geosciences IBG‐1: Biotechnology Forschungszentrum Jülich Jülich Germany
Institute of Molecular Enzyme Technology (IMET) Heinrich Heine University Düsseldorf Düsseldorf Germany
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Anita Loeschcke corresponding
Heinrich Heine University Düsseldorf
Affiliation as printed
Institute of Molecular Enzyme Technology (IMET) Heinrich Heine University Düsseldorf Düsseldorf Germany
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