Maturing differentiated human pluripotent stem cells in vitro: methods and challenges
Development, vol. 150
Abstract
Human pluripotent stem cells (hPSCs), derived from individuals or genetically modified with disease-related mutations and variants, have revolutionised studies of human disease. Researchers are beginning to exploit the extraordinary potential of stem cell technology to screen for new drugs to treat intractable diseases, ideally without side-effects. However, a major problem is that the differentiated cell types on which these models are based are immature; they resemble fetal and not adult cells. Here, we discuss the nature and hurdles of hPSC maturation, using cardiomyocytes as an example. We review methods used to induce cardiomyocyte maturation in culture and consider remaining challenges for their integration into research on human disease and drug development pipelines.
Authors 4
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Murdoch Children's Research Institute · Novo Nordisk Foundation Center for Stem Cell Medicine
Affiliation as printed
Murdoch Children's Research Institute 2 , Melbourne 3052 , Australia
The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW) 3 , Copenhagen 2200 , Denmark
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Murdoch Children's Research Institute · Novo Nordisk Foundation Center for Stem Cell Medicine
Affiliation as printed
Murdoch Children's Research Institute 2 , Melbourne 3052 , Australia
The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW) 3 , Copenhagen 2200 , Denmark
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Milena Bellin Aachen
University of Padua · Leiden University Medical Center · Veneto Institute of Molecular Medicine · Novo Nordisk Foundation Center for Stem Cell Medicine
Affiliation as printed
Leiden University Medical Center 4 Departmant of Anatomy and Embryology , , Leiden 2333ZC , The Netherlands
The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW) 3 , Copenhagen 2200 , Denmark
University of Padova 1 Department of Biology , , Padova 35131 , Italy
Veneto Institute of Molecular Medicine 5 , Padova 35129 , Italy
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Christine L. Mummery Aachen
Leiden University Medical Center · Novo Nordisk Foundation Center for Stem Cell Medicine · University of Twente
Affiliation as printed
Leiden University Medical Center 4 Departmant of Anatomy and Embryology , , Leiden 2333ZC , The Netherlands
The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW) 3 , Copenhagen 2200 , Denmark
University of Twente 6 Department of Applied Stem Cell Technologies , , Enschede 7522 , The Netherlands
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