Seed Longevity is Controlled by Metacaspases
bioRxiv (Cold Spring Harbor Laboratory)
Abstract
Abstract To survive extreme desiccation, seeds enter dormancy that can last millennia. This dormancy involves the accumulation of protective but structurally disordered storage proteins through unknown adjustments of proteolytic surveillance mechanisms. Mutation of all six types II metacaspases (MCAs)-II in the model plant Arabidopsis revealed their essential role in modulating these proteolytic mechanisms. MCA-II mutant seeds fail to properly target at the endoplasmic reticulum (ER) the AAA ATPase Cell Division Cycle 48 (CDC48) to dispose of misfolded proteins. MCA-IIs cleave a CDC48 adaptor, the ubiquitination regulatory X domain-containing (PUX) responsible for localizing CDC48 to the lipid droplets. When cleaved, CDC48-PUX is inactivated and allows a lipid droplet-to-ER shuttling of CDC48, an important step in the regulation of seeds’ lifespan. In sum, we uncover antagonism between proteolytic pathways bestowing longevity. One-Sentence Summary Metacaspase proteases confer seed longevity by antagonizing CDC48 activity.
Authors 19
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University of Crete · Swedish University of Agricultural Sciences
Affiliation as printed
Department of Biology, University of Crete, Heraklion, Greece
Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala, Sweden
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University of Crete · FORTH Institute of Molecular Biology and Biotechnology · Foundation for Research and Technology Hellas
Affiliation as printed
Department of Biology, University of Crete, Heraklion, Greece
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology – Hellas, Heraklion, Greece
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University of Potsdam · HMU Health and Medical University
Affiliation as printed
Department of Medicine, Health and Medical University, Potsdam, Germany
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Affiliation as printed
Plant Ecology and Evolution, Department of Ecology and Genetics, Evolutionary Biology Centre and the Linnean Centre for Plant Biology in Uppsala, Uppsala University, Uppsala, Sweden
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Swedish University of Agricultural Sciences
Affiliation as printed
Department of Molecular Sciences, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala, Sweden
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Swedish University of Agricultural Sciences
Affiliation as printed
Department of Molecular Sciences, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala, Sweden
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Swedish University of Agricultural Sciences · Ghent University · VIB-UGent Center for Plant Systems Biology
Affiliation as printed
Department of Molecular Sciences, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala, Sweden
Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium
VIB-Ugent Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium
VIB-Ugent Center for Plant Systems Biology, Technologiepark 1, 9052 Ghent, Belgium
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Consejo Superior de Investigaciones Científicas · Instituto de Bioquímica Vegetal y Fotosíntesis · Universidad de Sevilla
Affiliation as printed
Departamento de Bioquımica Vegetal y Biologıa Molecular, Facultad de Biologıa, Universidad de Sevilla, Sevilla, Spain
Instituto de Bioquımica Vegetal y Fotosıntesis, Consejo Superior de Investigaciones Cientıficas (CSIC)-Universidad de Sevilla, Sevilla, Spain
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Ghent University · VIB-UGent Center for Plant Systems Biology
Affiliation as printed
Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium
VIB-Ugent Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium
VIB-Ugent Center for Plant Systems Biology, Technologiepark 1, 9052 Ghent, Belgium
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Affiliation as printed
University of Bonn, Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), Karlrobert-Kreiten-Straße 13, 53115 Bonn, Germany
University of Bonn, Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), Karlrobert-Kreiten-Straße 13, 535 Bonn, Germany
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Centre National de la Recherche Scientifique · AgroParisTech · Université Paris-Saclay · Institut Jean-Pierre Bourgin
Affiliation as printed
Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, 78000 Versailles, France
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Ghent University · VIB-UGent Center for Plant Systems Biology
Affiliation as printed
Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium
VIB-Ugent Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium
VIB-Ugent Center for Plant Systems Biology, Technologiepark 1, 9052 Ghent, Belgium
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Ghent University · VIB-UGent Center for Plant Systems Biology
Affiliation as printed
Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium
VIB-Ugent Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium
VIB-Ugent Center for Plant Systems Biology, Technologiepark 1, 9052 Ghent, Belgium
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Affiliation as printed
Institute of Biology Leiden, Leiden University, Leiden 2333 BE, The Netherlands
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Ghent University · VIB-UGent Center for Plant Systems Biology
Affiliation as printed
Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium
VIB-Ugent Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium
VIB-Ugent Center for Plant Systems Biology, Technologiepark 1, 9052 Ghent, Belgium
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Ghent University · VIB-UGent Center for Plant Systems Biology
Affiliation as printed
Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium
VIB-Ugent Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium
VIB-Ugent Center for Plant Systems Biology, Technologiepark 1, 9052 Ghent, Belgium
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University of East Anglia · Sainsbury Laboratory
Affiliation as printed
The Sainsbury Laboratory, University of East Anglia, NR4 7UH Norwich, United Kingdom
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Swedish University of Agricultural Sciences
Affiliation as printed
Department of Molecular Sciences, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala, Sweden
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Panagiotis N. Moschou corresponding
University of Crete · Swedish University of Agricultural Sciences · FORTH Institute of Molecular Biology and Biotechnology · Foundation for Research and Technology Hellas
Affiliation as printed
Department of Biology, University of Crete, Heraklion, Greece
Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala, Sweden
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology – Hellas, Heraklion, Greece
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