Ad26.COV2.S priming provided a solid immunological base for mRNA-based COVID-19 booster vaccination
iScience, vol. 26, pp. 105753
Abstract
The emergence of novel SARS-CoV-2 variants led to the recommendation of booster vaccinations after Ad26.COV2.S priming. It was previously shown that heterologous booster vaccination induces high antibody levels, but how heterologous boosters affect other functional aspects of the immune response remained unknown. Here, we performed immunological profiling of Ad26.COV2.S-primed individuals before and after homologous or heterologous (mRNA-1273 or BNT162b2) booster. Booster vaccinations increased functional antibodies targeting ancestral SARS-CoV-2 and emerging variants. Especially heterologous booster vaccinations induced high levels of functional antibodies. In contrast, T-cell responses were similar in magnitude following homologous or heterologous booster vaccination and retained cross-reactivity towards variants. Booster vaccination led to a minimal expansion of SARS-CoV-2-specific T-cell clones and no increase in the breadth of the T-cell repertoire. In conclusion, we show that Ad26.COV2.S priming vaccination provided a solid immunological base for heterologous boosting, increasing humoral and cellular responses targeting emerging variants of concern.
Authors 24
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Affiliation as printed
Department of Viroscience, Erasmus Medical Center, Rotterdam, the Netherlands
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Affiliation as printed
Department of Hospital Pharmacy, Erasmus Medical Center, Rotterdam, the Netherlands
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University Medical Center Groningen · University of Groningen · National Institute for Public Health and the Environment
Affiliation as printed
Center for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, the Netherlands
Department of Medical Microbiology and Infection Prevention, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands
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Amsterdam University Medical Centers · University of Amsterdam
Affiliation as printed
Department of Experimental Immunology, Amsterdam University Medical Centers, Amsterdam Infection and Immunity Institute, University of Amsterdam, Amsterdam, the Netherlands
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Affiliation as printed
Department of Hospital Pharmacy, Erasmus Medical Center, Rotterdam, the Netherlands
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Affiliation as printed
Department of Viroscience, Erasmus Medical Center, Rotterdam, the Netherlands
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Affiliation as printed
Department of Viroscience, Erasmus Medical Center, Rotterdam, the Netherlands
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Affiliation as printed
Department of Viroscience, Erasmus Medical Center, Rotterdam, the Netherlands
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Affiliation as printed
Department of Viroscience, Erasmus Medical Center, Rotterdam, the Netherlands
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Affiliation as printed
Department of Viroscience, Erasmus Medical Center, Rotterdam, the Netherlands
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Affiliation as printed
Department of Hospital Pharmacy, Erasmus Medical Center, Rotterdam, the Netherlands
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Affiliation as printed
Department of Internal Medicine, Erasmus Medical Center, Rotterdam, the Netherlands
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Affiliation as printed
Department of Internal Medicine, Division of Allergy & Clinical Immunology and Department of Immunology, Erasmus Medical Center, Rotterdam, the Netherlands
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Amsterdam Public Health · Amsterdam University Medical Centers · University of Amsterdam
Affiliation as printed
Center of Tropical Medicine and Travel Medicine, Department of Infectious Diseases, Amsterdam University Medical Centers, Amsterdam, the Netherlands
Infection & Immunity, Amsterdam Public Health, University of Amsterdam, Amsterdam, the Netherlands
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University Medical Center Groningen
Affiliation as printed
Department of Internal Medicine and Infectious Diseases, University Medical Center Groningen, Groningen, the Netherlands
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Leiden University Medical Center
Affiliation as printed
Department of Infectious Diseases, Leiden University Medical Center, Leiden, the Netherlands
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University Medical Center Groningen · University of Groningen
Affiliation as printed
Department of Medical Microbiology and Infection Prevention, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands
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La Jolla Institute for Immunology · University of California San Diego
Affiliation as printed
Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology (LJI), La Jolla, CA, USA
Department of Medicine, Division of Infectious Diseases and Global Public Health, University of California, San Diego (UCSD), La Jolla, La Jolla, CA, USA
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La Jolla Institute for Immunology
Affiliation as printed
Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology (LJI), La Jolla, CA, USA
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Affiliation as printed
Department of Viroscience, Erasmus Medical Center, Rotterdam, the Netherlands
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Affiliation as printed
Department of Viroscience, Erasmus Medical Center, Rotterdam, the Netherlands
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Affiliation as printed
Department of Hospital Pharmacy, Erasmus Medical Center, Rotterdam, the Netherlands
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Affiliation as printed
Department of Viroscience, Erasmus Medical Center, Rotterdam, the Netherlands
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Rory D. de Vries corresponding
Affiliation as printed
Department of Viroscience, Erasmus Medical Center, Rotterdam, the Netherlands
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