Lipid profiling analyses from mouse models and human infants

STAR Protocols, vol. 3, pp. 101679

Abstract

This protocol outlines a translational lipidomic approach to discover lipid biomarkers that could predict morphometric body and histological organ measurements (e.g., weight and adiposity gains) during specific stages of life (e.g., early life). We describe procedures ranging from animal experimentation and histological analyses to downstream analytical steps through lipid profiling, both in mice and humans. This protocol represents a reliable and versatile approach to translate and validate candidate lipid biomarkers from animal models to a human cohort. For complete details on the use and execution of this protocol, please refer to Olga et al. (2021).

Authors 9

1. Laurentya Olga

   University of Cambridge

   Affiliation as printed

   Department of Paediatrics, University of Cambridge, Cambridge CB2 0QQ, UK

2. Ivana Bobeldijk‐Pastorova

   Netherlands Organisation for Applied Scientific Research

   Affiliation as printed

   Department of Metabolic Health Research, The Netherlands Organization for Applied Scientific Research (TNO), 2333 CK Leiden, the Netherlands

3. Richard C. Bas
   Affiliation as printed

   DUCARES B.V. | trading as TRISKELION, Reactorweg 47-A, 3542 AD Utrecht, the Netherlands

4. Florine Seidel
   Affiliation as printed

   DUCARES B.V. | trading as TRISKELION, Reactorweg 47-A, 3542 AD Utrecht, the Netherlands

5. Stuart G. Snowden

   University of Cambridge · Wellcome/MRC Institute of Metabolic Science

   Affiliation as printed

   Core Metabolomics and Lipidomics Laboratory, Metabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge Biomedical Campus, Cambridge CB2 0QQ, UK

6. Samuel Furse

   University of Cambridge · Wellcome/MRC Institute of Metabolic Science

   Affiliation as printed

   Core Metabolomics and Lipidomics Laboratory, Metabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge Biomedical Campus, Cambridge CB2 0QQ, UK

7. Ken K. Ong

   University of Cambridge · Wellcome/MRC Institute of Metabolic Science · MRC Epidemiology Unit · Wellcome Trust

   Affiliation as printed

   Department of Paediatrics, University of Cambridge, Cambridge CB2 0QQ, UK; MRC Epidemiology Unit, Wellcome Trust-MRC Institute of Metabolic Science, NIHR Cambridge Comprehensive Biomedical Research Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge CB2 0SL, UK; Institute of Metabolic Science, University of Cambridge, Cambridge CB2 0QQ, UK

   Department of Paediatrics, University of Cambridge, Cambridge CB2 0QQ, UK

   Institute of Metabolic Science, University of Cambridge, Cambridge CB2 0QQ, UK

   MRC Epidemiology Unit, Wellcome Trust-MRC Institute of Metabolic Science, NIHR Cambridge Comprehensive Biomedical Research Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge CB2 0SL, UK

8. Robert Kleemann Aachen Department of Vascular Surgery

   Leiden University Medical Center

   Affiliation as printed

   Department of Vascular Surgery, Leiden University Medical Center, 2333 ZA Leiden, the Netherlands

9. Albert Koulman corresponding

   University of Cambridge · National Institute for Health and Care Research · Wellcome/MRC Institute of Metabolic Science · MRC Epidemiology Unit · NIHR Cambridge Biomedical Research Centre · Wellcome Trust

   Affiliation as printed

   Core Metabolomics and Lipidomics Laboratory, Metabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge Biomedical Campus, Cambridge CB2 0QQ, UK; MRC Epidemiology Unit, Wellcome Trust-MRC Institute of Metabolic Science, NIHR Cambridge Comprehensive Biomedical Research Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge CB2 0SL, UK; Institute of Metabolic Science, University of Cambridge, Cambridge CB2 0QQ, UK. Electronic address: ak675@medschl.cam.ac.uk

   Core Metabolomics and Lipidomics Laboratory, Metabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge Biomedical Campus, Cambridge CB2 0QQ, UK

   Institute of Metabolic Science, University of Cambridge, Cambridge CB2 0QQ, UK

   MRC Epidemiology Unit, Wellcome Trust-MRC Institute of Metabolic Science, NIHR Cambridge Comprehensive Biomedical Research Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge CB2 0SL, UK