The minimum information required for a glycomics experiment (MIRAGE): reporting guidelines for capillary electrophoresis
Glycobiology, vol. 32, pp. 580–587
Abstract
The Minimum Information Required for a Glycomics Experiment (MIRAGE) is an initiative to standardize the reporting of glycoanalytical methods and to assess their reproducibility. To date, the MIRAGE Commission has published several reporting guidelines that describe what information should be provided for sample preparation methods, mass spectrometry methods, liquid chromatography analysis, exoglycosidase digestions, glycan microarray methods, and nuclear magnetic resonance methods. Here, we present the first version of reporting guidelines for glyco(proteo)mics analysis by capillary electrophoresis (CE) for standardized and high-quality reporting of experimental conditions in the scientific literature. The guidelines cover all aspects of a glyco(proteo)mics CE experiment including sample preparation, CE operation mode (CZE, CGE, CEC, MEKC, cIEF, cITP), instrument configuration, capillary separation conditions, detection, data analysis, and experimental descriptors. These guidelines are linked to other MIRAGE guidelines and are freely available through the project website https://www.beilstein-institut.de/en/projects/mirage/guidelines/#ce_analysis (doi:10.3762/mirage.7).
Authors 6
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Leiden University Medical Center
Affiliation as printed
Center for Proteomics and Metabolomics, Leiden University Medical Center , Albinusdreef 2, 2300 RA Leiden, Netherlands
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Zentrum für Innovation und Technik in Nordrhein-Westfalen · Max Planck Institute for Dynamics of Complex Technical Systems
Affiliation as printed
Max Planck Institute for Dynamics of Complex Technical Systems , Sandtorstraße 1, 39106 Magdeburg, Germany
glyXera GmbH , Brenneckestrasse 20—ZENIT, 39120 Magdeburg, Germany
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Affiliation as printed
Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University Medical Campus , 715 Albany Street, Boston, MA 02118, United States
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Agency for Science, Technology and Research · Bioprocessing Technology Institute
Affiliation as printed
Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR) , 20 Biopolis Way, 138668 Centros, Singapore
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Carsten Kettner corresponding
Affiliation as printed
Beilstein-Institut , Trakehner Str. 7-9, 60487 Frankfurt am Main, Germany
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Affiliation as printed
Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University Medical Campus , 715 Albany Street, Boston, MA 02118, United States
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