Recording Neuronal Activity On Chip with Segmented 3D Microelectrode Arrays

Proceedings, IEEE micro electro mechanical systems, pp. 102–105

Abstract

We present preliminary recordings on chip of three-dimensional (3D) electric neuronal activity from cultures of cortical neurons derived from human-induced pluripotent stem cells (hiPSCs). The recordings were obtained through 3D microelectrode arrays (MEAs) composed of truncated, 90 μm-high Si micropyramids endowed with multiple, electrically distinct, and vertically arranged TiN microelectrodes. The unique design and implementation of the 3D microelectrodes, complemented by a 60-electrode readout interface, allow for 3D spatial recording of neuronal activity, as well as single-unit recordings in high throughput, which are currently not possible with commercial MEA platforms. Future work will aim at optimizing extended 3D MEAs over optically transparent substrates for electro-physiological investigation of 3D neuronal tissues and organoids.

Authors 7

1. Nele Revyn

   Delft University of Technology

   Affiliation as printed

   TU Delft,ECTM,Department of Microelectronics,THE NETHERLANDS

   Department of Microelectronics, ECTM, TU Delft, THE NETHERLANDS

2. Michel H. Y. Hu Aachen Human Genetics and Neurology

   Leiden University · Leiden University Medical Center

   Affiliation as printed

   Human Genetics and Neurology, Leiden University Medical Centre,THE NETHERLANDS

   Human Genetics and Neurology, Leiden University Medical Centre, THE NETHERLANDS

3. J.M.S. Frimat Aachen Human Genetics and Neurology

   Leiden University · Leiden University Medical Center

   Affiliation as printed

   Human Genetics and Neurology, Leiden University Medical Centre,THE NETHERLANDS

   Human Genetics and Neurology, Leiden University Medical Centre, THE NETHERLANDS

4. Bjorn de Wagenaar

   Delft University of Technology

   Affiliation as printed

   TU Delft,ECTM,Department of Microelectronics,THE NETHERLANDS

   Department of Microelectronics, ECTM, TU Delft, THE NETHERLANDS

5. Arn M. J. M. van den Maagdenberg Aachen Human Genetics and Neurology

   Leiden University · Leiden University Medical Center

   Affiliation as printed

   Human Genetics and Neurology, Leiden University Medical Centre,THE NETHERLANDS

   Human Genetics and Neurology, Leiden University Medical Centre, THE NETHERLANDS

6. Pasqualina Maria Sarro

   Delft University of Technology

   Affiliation as printed

   TU Delft,ECTM,Department of Microelectronics,THE NETHERLANDS

   Department of Microelectronics, ECTM, TU Delft, THE NETHERLANDS

7. Massimo Mastrangeli

   Delft University of Technology

   Affiliation as printed

   TU Delft,ECTM,Department of Microelectronics,THE NETHERLANDS

   Department of Microelectronics, ECTM, TU Delft, THE NETHERLANDS