Immobilized-enzyme reactors integrated into analytical platforms: Recent advances and challenges
TrAC Trends in Analytical Chemistry, vol. 144, pp. 116419
Abstract
Immobilized-enzyme reactors (IMERs) are flow-through devices containing enzymes that are physically confined or localized with retention of their catalytic activities. IMERs can be used repeatedly and continuously and have been applied for (bio)polymer degradation, proteomics, biomarker discovery, inhibitor screening, and detection. Online integration of IMERs with analytical instrumentation, such as high-performance liquid chromatography (HPLC) systems, reduces the time needed for multi-step workflows, reduces the need for sample handling, and enables automation. However, online integration can also be challenging, as reaching its full potential requires complex instrumental setups and experienced users. This review aims to provide an assessment of recent advances and challenges in online IMER-based (analytical) LC platforms, covering publications from 2014–2021. A critical discussion of challenges often encountered in IMER fabrication, sample preparation, integration into the analytical workflow, long-term usage, and of potential ways to overcome these is provided. Finally, the obstacles preventing the proliferation of IMERs as efficient tools for high-throughput pharmacological, industrial, and biological studies are discussed.
Authors 5
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Bert Wouters corresponding Aachen Analytical Biosciences and Metabolomics Leiden Academic Centre for Drug Research
Affiliation as printed
Analytical Biosciences and Metabolomics, Systems Biomedicine and Pharmacology, Leiden Academic Centre for Drug Research, Leiden University, Einsteinweg 55, 2333, CC Leiden, the Netherlands
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Technological University Dublin
Affiliation as printed
Technological University Dublin, Tallaght Campus, Department of Applied Science, Dublin 24, Ireland
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Affiliation as printed
University of Amsterdam, van ’t Hoff Institute for Molecular Science (HIMS), Amsterdam, the Netherlands
University of Amsterdam, van 't Hoff Institute for Molecular Science (HIMS), Amsterdam, the Netherlands
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Thomas Hankemeier Aachen Analytical Biosciences and Metabolomics Leiden Academic Centre for Drug Research
Affiliation as printed
Analytical Biosciences and Metabolomics, Systems Biomedicine and Pharmacology, Leiden Academic Centre for Drug Research, Leiden University, Einsteinweg 55, 2333, CC Leiden, the Netherlands
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Affiliation as printed
University of Amsterdam, van ’t Hoff Institute for Molecular Science (HIMS), Amsterdam, the Netherlands
University of Amsterdam, van 't Hoff Institute for Molecular Science (HIMS), Amsterdam, the Netherlands
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