In Vitro Compression Model for Orthodontic Tooth Movement Modulates Human Periodontal Ligament Fibroblast Proliferation, Apoptosis and Cell Cycle

Biomolecules, vol. 11, pp. 932

Abstract

Human Periodontal Ligament Fibroblasts (hPDLF), as part of the periodontal apparatus, modulate inflammation, regeneration and bone remodeling. Interferences are clinically manifested as attachment loss, tooth loosening and root resorption. During orthodontic tooth movement (OTM), remodeling and adaptation of the periodontium is required in order to enable tooth movement. hPDLF involvement in the early phase-OTM compression side was investigated for a 72-h period through a well-studied in vitro model. Changes in the morphology, cell proliferation and cell death were analyzed. Specific markers of the cell cycle were investigated by RT-qPCR and Western blot. The study showed that the morphology of hPDLF changes towards more unstructured, unsorted filaments under mechanical compression. The total cell numbers were significantly reduced with a higher cell death rate over the whole observation period. hPDLF started to recover to pretreatment conditions after 48 h. Furthermore, key molecules involved in the cell cycle were significantly reduced under compressive force at the gene expression and protein levels. These findings revealed important information for a better understanding of the preservation and remodeling processes within the periodontium through Periodontal Ligament Fibroblasts during orthodontic tooth movement. OTM initially decelerates the hPDLF cell cycle and proliferation. After adapting to environmental changes, human Periodontal Ligament Fibroblasts can regain homeostasis of the periodontium, affecting its reorganization.

Authors 8

1. Julia Brockhaus Aachen Department of Orthodontics, Dental Clinic

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Department of Orthodontics, Dental Clinic, University Hospital RWTH Aachen, 52074 Aachen, Germany

2. Rogerio Bastos Craveiro corresponding Aachen Department of Orthodontics, Dental Clinic

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Department of Orthodontics, Dental Clinic, University Hospital RWTH Aachen, 52074 Aachen, Germany

3. Irma Azraq Aachen Department of Orthodontics, Dental Clinic

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Department of Orthodontics, Dental Clinic, University Hospital RWTH Aachen, 52074 Aachen, Germany

4. Christian Niederau Aachen Department of Orthodontics, Dental Clinic

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Department of Orthodontics, Dental Clinic, University Hospital RWTH Aachen, 52074 Aachen, Germany

5. Sarah K. Schröder Aachen Institute of Molecular Pathobiochemistry Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC)

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry, University Hospital RWTH Aachen, 52074 Aachen, Germany

6. Ralf Weiskirchen Aachen Institute of Molecular Pathobiochemistry Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC)

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry, University Hospital RWTH Aachen, 52074 Aachen, Germany

7. Joachim Jankowski Aachen Institute for Molecular Cardiovascular Research

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Institute for Molecular Cardiovascular Research, University Hospital RWTH Aachen, 52074 Aachen, Germany

8. Michael F. Wolf Aachen Department of Orthodontics, Dental Clinic

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Department of Orthodontics, Dental Clinic, University Hospital RWTH Aachen, 52074 Aachen, Germany