Lipocalin 2 as a potential systemic biomarker for central serous chorioretinopathy

Scientific Reports, vol. 10, pp. 20175

Abstract

No systemic biomarker of Central Serous Chorioretinopathy (CSCR) has been identified. Lipocalin 2 (LCN2 or NGAL), alone or complexed with MMP-9 (NGAL/MMP-9), is increased in several retinal disorders. Serum levels of LCN2 and NGAL/MMP-9 were measured in CSCR patients (n = 147) with chronic (n = 76) or acute/recurrent disease (n = 71) and in age- and sex-matched healthy controls (n = 130). Samples with CRP > 5 mg/L, creatinine > 100 µmol/L, and/or urea > 7.5 mmol/L were excluded. Serum LCN2 was lower in CSCR patients than controls (81.4 ± 48.7 vs 107.3 ± 44.5 ng/ml, p < 0.0001), and lower in acute/recurrent CSCR than controls (p < 0.001) and chronic CSCR (p = 0.006). Serum NGAL/MMP-9 was lower in CSCR patients than controls (47.2 ± 40.7 vs 74.1 ± 42.6, p < 0.0001), and lower in acute/recurrent CSCR than controls (p < 0.001) and chronic CSCR (p = 0.002). A ROC curve showed that for LCN2 serum levels, the 80-ng/ml cutoff value allows to discriminate acute/recurrent CSCR from controls with 80.3% sensitivity and 75.8% specificity, and for NGAL/MMP-9 serum levels, a 38-ng/ml cutoff value allows to discriminate acute/recurrent CSCR from controls with 69.6% sensitivity and 80.3% specificity. In both acute and chronic CSCR, low serum LCN2 and NGAL/MMP-9, provide a biological link between the two CSCR forms, and potential susceptibility to oxidative stress and innate immune dysregulation in CSCR.

Authors 13

1. Alexandre Matet

   Inserm · Université Paris Cité · Sorbonne Paris Cité · Centre de Recherche des Cordeliers · Institut Curie

   Affiliation as printed

   Centre de Recherche Des Cordeliers Inserm, UMR_1138, Physiopathology of Ocular Diseaic Innovationsses/Therapeut, Université de Paris, Université Sorbonne Paris Cité, 15 rue de l'Ecole de Médecine, 75006, Paris, France

   Department of Ophthalmology, Institut Curie, Paris, France

   Université de Paris, Paris, France

2. Thara Jaworski

   Inserm · Université Paris Cité · Sorbonne Paris Cité · Centre de Recherche des Cordeliers

   Affiliation as printed

   Centre de Recherche Des Cordeliers Inserm, UMR_1138, Physiopathology of Ocular Diseaic Innovationsses/Therapeut, Université de Paris, Université Sorbonne Paris Cité, 15 rue de l'Ecole de Médecine, 75006, Paris, France

3. Elodie Bousquet

   Inserm · Université Paris Cité · Sorbonne Paris Cité · Centre de Recherche des Cordeliers · Hôpital Cochin · Assistance Publique – Hôpitaux de Paris

   Affiliation as printed

   Centre de Recherche Des Cordeliers Inserm, UMR_1138, Physiopathology of Ocular Diseaic Innovationsses/Therapeut, Université de Paris, Université Sorbonne Paris Cité, 15 rue de l'Ecole de Médecine, 75006, Paris, France

   Ophthalmopole, Cochin University Hospital, Assistance Publique-Hôpitaux de Paris, Paris, France

   Université de Paris, Paris, France

4. Jérémie Canonica

   Inserm · Université Paris Cité · Hôpital Ophtalmique Jules-Gonin · Sorbonne Paris Cité · Centre de Recherche des Cordeliers · University of Lausanne

   Affiliation as printed

   Centre de Recherche Des Cordeliers Inserm, UMR_1138, Physiopathology of Ocular Diseaic Innovationsses/Therapeut, Université de Paris, Université Sorbonne Paris Cité, 15 rue de l'Ecole de Médecine, 75006, Paris, France

   Jules-Gonin Eye Hospital, Department of Ophthalmology, University of Lausanne, Lausanne, Switzerland

5. Camille Gobeaux

   Hôpital Cochin

   Affiliation as printed

   Service de Diagnostic Biologique Automatisé, Cochin Teaching Hospital, Paris, France

6. Alejandra Daruich

   Hôpital Necker-Enfants Malades · Inserm · Université Paris Cité · Sorbonne Paris Cité · Centre de Recherche des Cordeliers · Assistance Publique – Hôpitaux de Paris

   Affiliation as printed

   Centre de Recherche Des Cordeliers Inserm, UMR_1138, Physiopathology of Ocular Diseaic Innovationsses/Therapeut, Université de Paris, Université Sorbonne Paris Cité, 15 rue de l'Ecole de Médecine, 75006, Paris, France

   Necker-Enfants Malades University Hospital, Department of Ophthalmology, Assistance Publique-Hôpitaux de Paris, Paris, France

   Université de Paris, Paris, France

7. Min Zhao

   Inserm · Université Paris Cité · Sorbonne Paris Cité · Centre de Recherche des Cordeliers

   Affiliation as printed

   Centre de Recherche Des Cordeliers Inserm, UMR_1138, Physiopathology of Ocular Diseaic Innovationsses/Therapeut, Université de Paris, Université Sorbonne Paris Cité, 15 rue de l'Ecole de Médecine, 75006, Paris, France

8. Marta Zola

   Inserm · Université Paris Cité · Hôpital Ophtalmique Jules-Gonin · Sorbonne Paris Cité · Centre de Recherche des Cordeliers · University of Lausanne

   Affiliation as printed

   Centre de Recherche Des Cordeliers Inserm, UMR_1138, Physiopathology of Ocular Diseaic Innovationsses/Therapeut, Université de Paris, Université Sorbonne Paris Cité, 15 rue de l'Ecole de Médecine, 75006, Paris, France

   Jules-Gonin Eye Hospital, Department of Ophthalmology, University of Lausanne, Lausanne, Switzerland

9. Magda A. Meester‐Smoor

   Erasmus MC

   Affiliation as printed

   Department of Ophthalmology, Erasmus MC, Rotterdam, The Netherlands

10. Danial Mohabati Aachen Department of Ophthalmology, Leiden University Medical Center

    Leiden University Medical Center · Rotterdam Eye Hospital

    Affiliation as printed

    Department of Ophthalmology, Leiden University Medical center, The Netherlands

    Rotterdam Ophthalmic Institute, Ophthalmic Institute, Rotterdam, The Netherlands

11. Frédéric Jaisser

    Inserm · Université Paris Cité · Sorbonne Paris Cité · Centre de Recherche des Cordeliers

    Affiliation as printed

    Centre de Recherche Des Cordeliers Inserm, UMR_1138, Physiopathology of Ocular Diseaic Innovationsses/Therapeut, Université de Paris, Université Sorbonne Paris Cité, 15 rue de l'Ecole de Médecine, 75006, Paris, France

12. Suzanne Yzer

    Rotterdam Eye Hospital

    Affiliation as printed

    Rotterdam Eye Hospital, Rotterdam, The Netherlands

    Rotterdam Ophthalmic Institute, Ophthalmic Institute, Rotterdam, The Netherlands

13. Francine Béhar‐Cohen corresponding

    Inserm · Université Paris Cité · Sorbonne Paris Cité · Centre de Recherche des Cordeliers · Hôpital Cochin · Assistance Publique – Hôpitaux de Paris

    Affiliation as printed

    Centre de Recherche Des Cordeliers Inserm, UMR_1138, Physiopathology of Ocular Diseaic Innovationsses/Therapeut, Université de Paris, Université Sorbonne Paris Cité, 15 rue de l'Ecole de Médecine, 75006, Paris, France. Francine.behar@gmail.com

    Ophthalmopole, Cochin University Hospital, Assistance Publique-Hôpitaux de Paris, Paris, France. Francine.behar@gmail.com

    Université de Paris, Paris, France. Francine.behar@gmail.com

    Université de Paris, Paris, France