In Situ Dynamic Light Scattering Complementing Neutron Spin Echo Measurements on Protein Samples

Journal of Surface Investigation X-ray Synchrotron and Neutron Techniques, vol. 14, pp. S185–S189

Abstract

Abstract Monitoring the state of the sample on the minute-time scale is crucial in case of sensitive soft matter or biological samples, given that neutron spin echo measurements take up to several days. Moreover, there is no method to interpret the normalized intermediate scattering function obtained by measuring the neutron spin echo if relevant sample properties change during measurement. Dynamic light scattering provides information on the diffusion constant of particles in solution (biological macromolecules, such as proteins, protein aggregates, polymer particles, etc.) with average hydrodynamic radii in a wide range from several nanometers up to several microns. This information can be obtained in a few minutes and it offers a good overview of the current sample state. Details on the novel in situ dynamic light scattering set-up with one fixed scattering angle and first results obtained on a molten globule state of apo-myoglobin are presented.

Authors 12

1. Livia Balacescu Aachen Physikalisches Institut

   Forschungszentrum Jülich · Heinz Maier-Leibnitz Zentrum · RWTH Aachen University

   Affiliation as printed

   Forschungszentrum Jülich, Jülich Centre for Neutron Science at Heinz Maier-Leibnitz Zentrum, 85748, Garching, Germany

   Physikalisches Institut, AG Biophysik, 52074, Rheinisch-Westfälische Technische Hochschule Aachen, Germany

2. Florian Vögl

   Forschungszentrum Jülich · Heinz Maier-Leibnitz Zentrum

   Affiliation as printed

   Forschungszentrum Jülich, Jülich Centre for Neutron Science at Heinz Maier-Leibnitz Zentrum, 85748, Garching, Germany

3. Simon Staringer

   Forschungszentrum Jülich · Heinz Maier-Leibnitz Zentrum

   Affiliation as printed

   Forschungszentrum Jülich, Jülich Centre for Neutron Science at Heinz Maier-Leibnitz Zentrum, 85748, Garching, Germany

4. Vladimir Ossovyi

   Forschungszentrum Jülich · Heinz Maier-Leibnitz Zentrum

   Affiliation as printed

   Forschungszentrum Jülich, Jülich Centre for Neutron Science at Heinz Maier-Leibnitz Zentrum, 85748, Garching, Germany

5. Georg Brandl

   Forschungszentrum Jülich · Heinz Maier-Leibnitz Zentrum

   Affiliation as printed

   Forschungszentrum Jülich, Jülich Centre for Neutron Science at Heinz Maier-Leibnitz Zentrum, 85748, Garching, Germany

6. N. Lumma

   Forschungszentrum Jülich

   Affiliation as printed

   Forschungszentrum Jülich, Jülich Centre for Neutron Science and Institute for Complex Systems, 52425, Jülich, Germany

7. Herbert Feilbach

   Forschungszentrum Jülich

   Affiliation as printed

   Forschungszentrum Jülich, Jülich Centre for Neutron Science and Institute for Complex Systems, 52425, Jülich, Germany

8. Olaf Holderer

   Forschungszentrum Jülich · Heinz Maier-Leibnitz Zentrum

   Affiliation as printed

   Forschungszentrum Jülich, Jülich Centre for Neutron Science at Heinz Maier-Leibnitz Zentrum, 85748, Garching, Germany

9. Stefano Pasini

   Forschungszentrum Jülich · Heinz Maier-Leibnitz Zentrum

   Affiliation as printed

   Forschungszentrum Jülich, Jülich Centre for Neutron Science at Heinz Maier-Leibnitz Zentrum, 85748, Garching, Germany

10. Andreas Maximilian Stadler

    Forschungszentrum Jülich

    Affiliation as printed

    Forschungszentrum Jülich, Jülich Centre for Neutron Science and Institute for Complex Systems, 52425, Jülich, Germany

11. Jörg Fitter Aachen Physikalisches Institut

    Forschungszentrum Jülich · RWTH Aachen University

    Affiliation as printed

    Forschungszentrum Jülich, Institute for Complex Systems (ICS-5), 52425, Jülich, Germany

    Physikalisches Institut, AG Biophysik, 52074, Rheinisch-Westfälische Technische Hochschule Aachen, Germany

12. Tobias E. Schrader corresponding

    Forschungszentrum Jülich · Heinz Maier-Leibnitz Zentrum

    Affiliation as printed

    Forschungszentrum Jülich, Jülich Centre for Neutron Science at Heinz Maier-Leibnitz Zentrum, 85748, Garching, Germany