Distinguish fatty acids impact survival, differentiation and cellular function of periodontal ligament fibroblasts

Scientific Reports, vol. 10, pp. 15706

Abstract

Alveolar bone (AB) remodeling is necessary for the adaption to mechanical stimuli occurring during mastication and orthodontic tooth movement (OTM). Thereby, bone degradation and assembly are strongly regulated processes that can be altered in obese patients. Further, increased fatty acids (FA) serum levels affect bone remodeling cells and we, therefore, investigated whether they also influence the function of periodontal ligament fibroblast (PdLF). PdLF are a major cell type regulating the differentiation and function of osteoblasts and osteoclasts localized in the AB. We stimulated human PdLF (HPdLF) in vitro with palmitic (PA) or oleic acid (OA) and analyzed their metabolic activity, growth, survival and expression of osteogenic markers and calcium deposits. Our results emphasize that PA increased cell death of HPdLF, whereas OA induced their osteoblastic differentiation. Moreover, quantitative expression analysis of OPG and RANKL revealed altered levels in mechanically stimulated PA-treated HPdLF. Furthermore, osteoclasts stimulated with culture medium of mechanical stressed FA-treated HPdLF revealed significant changes in cell differentiation upon FA-treatment. For the first time, our results highlight a potential role of specific FA in the function of HPdLF-modulated AB remodeling and help to elucidate the complex interplay of bone metabolism, mechanical stimulation and obesity-induced alterations.

Authors 11

1. Judit Symmank corresponding

   Jena University Hospital

   Affiliation as printed

   Department of Orthodontics, University Hospital Jena, Leutragraben 3, 07743, Jena, Germany. judit.symmank@med.uni-jena.de

   Department of Orthodontics, University Hospital Jena, Leutragraben 3, 07743, Jena, Germany

2. Martin Chorus Aachen Department of Orthodontics

   Universitätsklinikum Aachen · Jena University Hospital · RWTH Aachen University

   Affiliation as printed

   Department of Orthodontics, University Hospital Jena, Leutragraben 3, 07743, Jena, Germany

   Department of Orthodontics, University Hospital RWTH Aachen, Pauwelsstraße 30, 52074, Aachen, Germany

3. Sophie Appel Aachen Department of Orthodontics

   Universitätsklinikum Aachen · Jena University Hospital · RWTH Aachen University

   Affiliation as printed

   Department of Orthodontics, University Hospital Jena, Leutragraben 3, 07743, Jena, Germany

   Department of Orthodontics, University Hospital RWTH Aachen, Pauwelsstraße 30, 52074, Aachen, Germany

4. Jana Marciniak Aachen Department of Orthodontics

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Department of Orthodontics, University Hospital RWTH Aachen, Pauwelsstraße 30, 52074, Aachen, Germany

5. Isabel Knaup Aachen Department of Orthodontics

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Department of Orthodontics, University Hospital RWTH Aachen, Pauwelsstraße 30, 52074, Aachen, Germany

6. Asisa Bastian Aachen Department of Orthodontics

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Department of Orthodontics, University Hospital RWTH Aachen, Pauwelsstraße 30, 52074, Aachen, Germany

7. Christoph‐Ludwig Hennig

   Jena University Hospital

   Affiliation as printed

   Department of Orthodontics, University Hospital Jena, Leutragraben 3, 07743, Jena, Germany

8. Annika Döding

   Jena University Hospital

   Affiliation as printed

   Section of Geriodontics, Department of Conservative Dentistry and Periodontics, University Hospital Jena, Leutragraben 3, 07743, Jena, Germany

9. Ulrike Schulze‐Späte

   Jena University Hospital

   Affiliation as printed

   Section of Geriodontics, Department of Conservative Dentistry and Periodontics, University Hospital Jena, Leutragraben 3, 07743, Jena, Germany

10. Collin Jacobs

    Jena University Hospital

    Affiliation as printed

    Department of Orthodontics, University Hospital Jena, Leutragraben 3, 07743, Jena, Germany

11. Michael F. Wolf Aachen Department of Orthodontics

    Universitätsklinikum Aachen · RWTH Aachen University

    Affiliation as printed

    Department of Orthodontics, University Hospital RWTH Aachen, Pauwelsstraße 30, 52074, Aachen, Germany