A 3D microelectrode array to record neural activity at different tissue depths

Data Archiving and Networked Services (DANS)

Abstract

In vitro study of high-level neurobiological systems requires three-dimensional (3D) neuronal cultures [1]. Meas-uring responses along all three spatial dimensions is critical to record electric activity inside 3D neuronal models, such as organoids and other 3D brain tissue constructs. However, this lies beyond the capacity of 2D microelec-trode arrays (MEAs) [2]. We present planar arrays of 3D micro-pyramids, whereby each micro-pyramid supports multiple, electrically distinct and vertically stacked microelectrodes. The 3D microarrays were produced by wafer-scale micromachining and assembled onto printed circuit boards (PCBs) conforming to MEA readout standards.

Authors 6

1. T.M. de Rijk
2. Michel H. Y. Hu Aachen

   Leiden University Medical Center

   Affiliation as printed

   LEIDEN UNIVERSITY (MEDICAL CENTER)

3. Jean‐Philippe Frimat Aachen

   Leiden University Medical Center

   Affiliation as printed

   LEIDEN UNIVERSITY (MEDICAL CENTER)

4. Arn M. J. M. van den Maagdenberg Aachen

   Leiden University Medical Center

   Affiliation as printed

   LEIDEN UNIVERSITY (MEDICAL CENTER)

5. Pasqualina Maria Sarro
   Affiliation as printed

   Electronic Components, Technology and Materials

6. Massimo Mastrangeli
   Affiliation as printed

   Electronic Components, Technology and Materials