Blood collection technique, anticoagulants and storing temperature have minor effects on the isolation of polymorphonuclear neutrophils

Scientific Reports, vol. 10, pp. 14646

Abstract

In the isolation of polymorphonuclear neutrophils (PMNs) the technique and other external factors can have great influence on the quality and quantity of isolated neutrophils. To elucidate the influence of the blood collection technique, anticoagulants and storing temperature on isolated PMNs healthy volunteers provided blood samples with different needles and collection techniques, anticoagulants (EDTA, heparin, citrate) and storing temperatures (4, 22, 37 °C). From each blood sample PMNs were isolated and compared regarding number of PMNs and oxidative burst. The blood collection technique, anticoagulants and storing temperature had minor impact on isolated PMNs. All three tested cannulas and anticoagulants can be used to obtain blood samples for PMN isolation. For storing temperatures 37 °C should be preferred. Regarding time between the PMN isolation and the actual experiments, a time span of maximum 1 h should be targeted.

Authors 7

1. Julia Krabbe corresponding Aachen Medical Faculty Institute of Occupational, Social and Environmental Medicine

   RWTH Aachen University

   Affiliation as printed

   Institute of Occupational, Social and Environmental Medicine, Medical Faculty, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany. jkrabbe@ukaachen.de

   Institute of Occupational, Social and Environmental Medicine, Medical Faculty, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany

2. Viktor Beilmann Aachen Medical Faculty Institute of Pharmacology and Toxicology Institute of Occupational, Social and Environmental Medicine

   RWTH Aachen University

   Affiliation as printed

   Institute of Occupational, Social and Environmental Medicine, Medical Faculty, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany

   Institute of Pharmacology and Toxicology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany

3. Hanif Alamzad-Krabbe Aachen Medical Faculty Institute of Pharmacology and Toxicology

   RWTH Aachen University

   Affiliation as printed

   Institute of Pharmacology and Toxicology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany

4. Svenja Böll Aachen Medical Faculty Institute of Pharmacology and Toxicology Department of Pediatrics, Medical Faculty

   RWTH Aachen University · Universitätsklinikum Aachen

   Affiliation as printed

   Department of Pediatrics, Medical Faculty, RWTH Aachen University, University Hospital Aachen, Pauwelstraße 30, 52074, Aachen, Germany

   Institute of Pharmacology and Toxicology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany

5. Anke Seifert Aachen Medical Faculty Institute of Pharmacology and Toxicology

   RWTH Aachen University

   Affiliation as printed

   Institute of Pharmacology and Toxicology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany

6. Nadine Ruske Aachen Medical Faculty Institute of Pharmacology and Toxicology

   RWTH Aachen University

   Affiliation as printed

   Institute of Pharmacology and Toxicology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany

7. Christian Martin Aachen Medical Faculty Institute of Pharmacology and Toxicology

   RWTH Aachen University

   Affiliation as printed

   Institute of Pharmacology and Toxicology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany