Benzoate Synthesis from Glucose or Glycerol Using Engineered Pseudomonas taiwanensis

Biotechnology Journal, vol. 15, pp. e2000211

Abstract

Benzoic acid is one of the most commonly used food preservatives, but currently exclusively produced in petrochemical processes. In this study, a bio-based production pathway using an engineered strain of Pseudomonas taiwanensis is described. In a phenylalanine-overproducing strain, bacterial and plant genes are heterologously expressed to achieve production of benzoate via a β-oxidation pathway. Strategic disruption of the native Pseudomonas benzoate degradation pathway further allows the production of catechol and cis,cis-muconate. Taken together, this work demonstrates new routes for the microbial production of these industrially relevant chemicals from renewable resources.

Authors 5

1. Maike Otto

   Forschungszentrum Jülich

   Affiliation as printed

   Institute of Bio‐ and Geosciences (IBG‐1: Biotechnology) Forschungszentrum Jülich GmbH Jülich 52425 Germany

2. Benedikt Wynands

   Forschungszentrum Jülich

   Affiliation as printed

   Institute of Bio‐ and Geosciences (IBG‐1: Biotechnology) Forschungszentrum Jülich GmbH Jülich 52425 Germany

3. Jan Marienhagen Aachen Institute of Biotechnology

   Forschungszentrum Jülich · RWTH Aachen University

   Affiliation as printed

   Institute of Biotechnology RWTH Aachen University Aachen 52074 Germany

   Institute of Bio‐ and Geosciences (IBG‐1: Biotechnology) Forschungszentrum Jülich GmbH Jülich 52425 Germany

   Institute of Bio‐ and Geosciences (IBG‐1: Biotechnology) Forschungszentrum Jülich GmbH Jülich 52425 Germany

4. Lars M. Blank Aachen Institute of Applied Microbiology

   RWTH Aachen University

   Affiliation as printed

   Institute of Applied Microbiology RWTH Aachen University Aachen 52074 Germany

   Institute of Applied Microbiology, RWTH Aachen University, Aachen, 52074 Germany

5. Nick J. P. Wierckx corresponding

   Forschungszentrum Jülich

   Affiliation as printed

   Institute of Bio‐ and Geosciences (IBG‐1: Biotechnology) Forschungszentrum Jülich GmbH Jülich 52425 Germany