Fasting parameters for estimation of stimulated β cell function in islet transplant recipients with or without basal insulin treatment

American Journal of Transplantation, vol. 21, pp. 297–306

Abstract

In order to assess β cell secretory capacity after islet transplantation, standardized mixed meal stimulation tests are often used. But these tests are cumbersome and the effect of exogenous insulin on the test results is unclear. The aim of our study was to determine to what extent fasting glycemic indices can estimate stimulated β cell function in islet transplant recipients with and without basal insulin. In total 100 mixed meal stimulation tests, including 31 with concurrent basal insulin treatment, were performed in 36 islet transplant recipients. In a multivariate model, fasting C-peptide and fasting glucose together estimated peak C-peptide with R2 = .87 and area under the curve (AUC) C-peptide with a R2 = .93. There was a larger increase of glucose during tests in which exogenous insulin was used (+7.9 vs +5.3 mmol/L, P < .001) and exogenous insulin use was associated with a slightly lower estimated peak C-peptide (relative change: −15%, P = .02). In islet transplant recipients the combination of fasting C-peptide and glucose can be used to accurately estimate stimulated β cell function after a mixed meal stimulation test, whether exogenous basal insulin is present or not. These data indicate that graft function can be reliably determined during exogenous insulin treatment and that regular islet graft stimulation tests can be minimized.

Authors 4

1. Bas S. Uitbeijerse Aachen Department of Internal Medicine

   Leiden University Medical Center

   Affiliation as printed

   Department of Internal Medicine, Leiden University Medical Center, Leiden, the Netherlands

2. Michiel F. Nijhoff Aachen Department of Internal Medicine

   Leiden University Medical Center

   Affiliation as printed

   Department of Internal Medicine, Leiden University Medical Center, Leiden, the Netherlands

3. Jacob K. Sont Aachen Department of Biomedical Data Sciences Section Medical Decision Making

   Leiden University Medical Center

   Affiliation as printed

   Department of Biomedical Data Sciences, Section Medical Decision Making, Leiden University Medical Center, Leiden, the Netherlands

4. Eelco J.P. de Koning corresponding Aachen Department of Internal Medicine

   Leiden University Medical Center

   Affiliation as printed

   Department of Internal Medicine, Leiden University Medical Center, Leiden, the Netherlands