A Supramolecular Platform Technology for Bacterial Cell Surface Modification

ACS Infectious Diseases, vol. 6, pp. 1734–1744

Abstract

In an era of antimicrobial resistance, a better understanding of the interaction between bacteria and the sentinel immune system is needed to discover new therapeutic targets for combating bacterial infectious disease. Sentinel immune cells such as macrophages phagocytose intact bacteria and thereby initiate ensuing immune responses. The bacterial surface composition is a key element that determines the macrophage signaling. To study the role of the bacterial cell surface composition in immune recognition, we developed a platform technology for altering bacterial surfaces in a controlled manner with versatile chemical scaffolds. We show that these scaffolds are efficiently loaded onto both Gram-positive and -negative bacteria and that their presence does not impair the capacity of monocyte-derived macrophages to phagocytose bacteria and subsequently signal to other components of the immune system. We believe this technology thus presents a useful tool to study the role of bacterial cell surface composition in disease etiology and potentially in novel interventions utilizing intact bacteria for vaccination.

Authors 8

1. Nikolas Duszenko Aachen Department of Parasitology, Leiden University Medical Center Interventional Molecular Imaging Laboratory Department of Radiology, Leiden University Medical Center

   Leiden University · Leiden University Medical Center

   Affiliation as printed

   Leiden University Medical Center , , Albinusdreef 2 , ,

   Department of Parasitology, Leiden University Medical Center, Albinusdreef 2, Leiden 2333 ZA, The Netherlands

   Interventional Molecular Imaging Laboratory, Department of Radiology, Leiden University Medical Center, Albinusdreef 2, Leiden 2333 ZA, The Netherlands

2. Danny M. van Willigen Aachen Interventional Molecular Imaging Laboratory Department of Radiology, Leiden University Medical Center

   Leiden University · Leiden University Medical Center

   Affiliation as printed

   Leiden University Medical Center , , Albinusdreef 2 , ,

   Interventional Molecular Imaging Laboratory, Department of Radiology, Leiden University Medical Center, Albinusdreef 2, Leiden 2333 ZA, The Netherlands

3. Mick M. Welling Aachen Interventional Molecular Imaging Laboratory Department of Radiology, Leiden University Medical Center

   Leiden University · Leiden University Medical Center

   Affiliation as printed

   Leiden University Medical Center , , Albinusdreef 2 , ,

   Interventional Molecular Imaging Laboratory, Department of Radiology, Leiden University Medical Center, Albinusdreef 2, Leiden 2333 ZA, The Netherlands

4. Clarize M. de Korne Aachen Department of Parasitology, Leiden University Medical Center Interventional Molecular Imaging Laboratory Department of Radiology, Leiden University Medical Center

   Leiden University · Leiden University Medical Center

   Affiliation as printed

   Leiden University Medical Center , , Albinusdreef 2 , ,

   Department of Parasitology, Leiden University Medical Center, Albinusdreef 2, Leiden 2333 ZA, The Netherlands

   Interventional Molecular Imaging Laboratory, Department of Radiology, Leiden University Medical Center, Albinusdreef 2, Leiden 2333 ZA, The Netherlands

5. Roos van Schuijlenburg Aachen Department of Parasitology, Leiden University Medical Center

   Leiden University Medical Center

   Affiliation as printed

   Leiden University Medical Center , , Albinusdreef 2 , ,

   Department of Parasitology, Leiden University Medical Center, Albinusdreef 2, Leiden 2333 ZA, The Netherlands

6. Béatrice M. F. Winkel Aachen Department of Parasitology, Leiden University Medical Center

   Leiden University Medical Center

   Affiliation as printed

   Leiden University Medical Center , , Albinusdreef 2 , ,

   Department of Parasitology, Leiden University Medical Center, Albinusdreef 2, Leiden 2333 ZA, The Netherlands

7. Fijs W. B. van Leeuwen Aachen Interventional Molecular Imaging Laboratory Department of Radiology, Leiden University Medical Center

   Leiden University · Leiden University Medical Center · The Netherlands Cancer Institute

   Affiliation as printed

   Leiden University Medical Center , , Albinusdreef 2 , ,

   Netherlands Cancer Institute-Antoni van Leeuwenhoek Hospital , , ,

   Department of Urology, Netherlands Cancer Institute-Antoni van Leeuwenhoek Hospital, Amsterdam 1066 CX, The Netherlands

   Interventional Molecular Imaging Laboratory, Department of Radiology, Leiden University Medical Center, Albinusdreef 2, Leiden 2333 ZA, The Netherlands

8. Meta Roestenberg corresponding Aachen Department of Infectious Diseases Department of Parasitology, Leiden University Medical Center

   Leiden University Medical Center

   Affiliation as printed

   Leiden University Medical Center , , Albinusdreef 2 , ,

   Department of Infectious Diseases, Leiden University Medical Center, Albinusdreef 2, Leiden 2333 ZA, The Netherlands

   Department of Parasitology, Leiden University Medical Center, Albinusdreef 2, Leiden 2333 ZA, The Netherlands