Modular and Versatile Trans‐Encoded Genetic Switches

Angewandte Chemie International Edition, vol. 59, pp. 20328–20332

Abstract

Current bacterial RNA switches suffer from lack of versatile inputs and are difficult to engineer. We present versatile and modular RNA switches that are trans-encoded and based on tRNA-mimicking structures (TMSs). These switches provide a high degree of freedom for reengineering and can thus be designed to accept a wide range of inputs, including RNA, small molecules, and proteins. This powerful approach enables control of the translation of protein expression from plasmid and genome DNA.

Authors 4

1. Avishek Paul Aachen DWI - Leibniz Institute for Interactive Materials

   DWI – Leibniz Institute for Interactive Materials · University of Groningen · Zernike Institute for Advanced Materials

   Affiliation as printed

   DWI-Leibniz Institute for Interactive Materials Forckenbeckstr. 50 52056 Aachen Germany

   Zernike Institute for Advanced Materials University of Groningen Nijenborgh 4 9747 AG Groningen The Netherlands

   DWI-Leibniz Institute for Interactive Materials, Forckenbeckstr. 50, 52056 Aachen, Germany

   Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands

2. Eliza M. Warszawik

   University of Groningen · Zernike Institute for Advanced Materials

   Affiliation as printed

   Zernike Institute for Advanced Materials University of Groningen Nijenborgh 4 9747 AG Groningen The Netherlands

   Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands

3. Mark Loznik Aachen DWI - Leibniz Institute for Interactive Materials

   DWI – Leibniz Institute for Interactive Materials · University of Groningen · Zernike Institute for Advanced Materials

   Affiliation as printed

   DWI-Leibniz Institute for Interactive Materials Forckenbeckstr. 50 52056 Aachen Germany

   Zernike Institute for Advanced Materials University of Groningen Nijenborgh 4 9747 AG Groningen The Netherlands

   DWI-Leibniz Institute for Interactive Materials, Forckenbeckstr. 50, 52056 Aachen, Germany

   Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands

4. Arnold J. Boersma Aachen Institute of Technical and Macromolecular Chemistry DWI - Leibniz Institute for Interactive Materials

   RWTH Aachen University · DWI – Leibniz Institute for Interactive Materials

   Affiliation as printed

   DWI-Leibniz Institute for Interactive Materials Forckenbeckstr. 50 52056 Aachen Germany

   Institute of Technical and Macromolecular Chemistry RWTH Aachen University Worringerweg 2 52074 Aachen Germany

   DWI-Leibniz Institute for Interactive Materials, Forckenbeckstr. 50, 52056 Aachen, Germany

   Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Worringerweg 2, 52074 Aachen, Germany