NFDI4BIOIMAGE Calendar 2026 January

Zenodo (CERN European Organization for Nuclear Research)

Abstract

January image of the NFDI4BIOIMAGE wall calendar 2026, contributed by Sarah Knapp. The original image can be found in BioImage Archive: https://doi.org/10.6019/S-BIAD1673 . Image description: This image highlights PARP15.1, which forms distinct nuclear foci when its ADP-ribosyltransferase domain is active. It shows the reference state of a cell-based assay designed to monitor mono-ADPribosylation (MARylation)-driven foci. Viral macrodomains, such as those from Chikungunya virus and SARS-CoV-2, antagonize this process by reversing host MARylation resulting in spread nuclear GFP signal. Cells were imaged by live-cell confocal microscopy. Channel 1: GFP from mEGFP-PARP15.1. Channel 2: brightfield showing morphology and cell health. Channel 3: nuclear DNA stained with Spy650DNA.

Authors 2

1. Sarah Knapp Aachen Institute of Biochemistry and Molecular Biology Medical Faculty

   RWTH Aachen University

   Affiliation as printed

   Institute of Biochemistry and Molecular Biology, Medical Faculty, RWTH Aachen University

2. Patricia Korn Aachen Institute of Biochemistry and Molecular Biology Medical Faculty

   RWTH Aachen University

   Affiliation as printed

   Institute of Biochemistry and Molecular Biology, Medical Faculty, RWTH Aachen University