Type 2 diabetes reshapes the B-cell compartment in tuberculosis, with reduction of transitional B cells and expansion of plasmablasts
International Journal of Infectious Diseases, vol. 172, pp. 109083
Abstract
OBJECTIVE: Type 2 diabetes (T2D) increases the risk of tuberculosis (TB) and TB severity; however, its effects on B-cells in the TB-T2D syndemic are not fully understood. METHODS: We evaluated by flow cytometry, the distribution of circulating B-cell subsets in patients with TB disease, TB-T2D, subjects with TB infection (TBI), TBI-T2D, healthy controls (HCs), T2D, patients with pulmonary respiratory diseases other than TB (ORDs), and ORD-T2D. Plasma levels of unspecific or PPD-specific IgG and B-cell-related soluble factors were measured by multiplex assays or ELISA. RESULTS: Total B-cell frequency was similar across groups. TB-T2D patients showed decreased transitional B cells compared to TB, TBI-T2D, HC, and T2D. Transitional B-cell frequency significantly and negatively correlated with HbA1c levels in the TB-T2D group. Moreover, plasmablast frequency was increased in TB-T2D compared to TB, TBI-T2D, and T2D. Unspecific or PPD-specific IgG levels were not modulated by T2D. BAFF levels increased in TB and TB-T2D compared to the other groups, whereas the levels of SDF-1 were decreased in TB and TBI irrespective of T2D, compared to the controls. CONCLUSIONS: Overall, T2D reshapes B-cell compartments in TB, reducing potentially anti-inflammatory transitional B-cells and increasing plasmablasts, possibly reinforcing inflammation and impacting B-cell development and humoral immunity in TB-T2D.
Authors 18
-
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani · Istituti di Ricovero e Cura a Carattere Scientifico
Affiliation as printed
Translational Research Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy
-
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani · Istituti di Ricovero e Cura a Carattere Scientifico
Affiliation as printed
Translational Research Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy
-
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani · Istituti di Ricovero e Cura a Carattere Scientifico
Affiliation as printed
Translational Research Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy
-
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani · Istituti di Ricovero e Cura a Carattere Scientifico
Affiliation as printed
Translational Research Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy
-
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani · Istituti di Ricovero e Cura a Carattere Scientifico
Affiliation as printed
Translational Research Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy
-
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani · Istituti di Ricovero e Cura a Carattere Scientifico
Affiliation as printed
Translational Research Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy
-
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani · Istituti di Ricovero e Cura a Carattere Scientifico
Affiliation as printed
Translational Research Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy
-
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani · Istituti di Ricovero e Cura a Carattere Scientifico
Affiliation as printed
Translational Research Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy; Professioni Sanitarie Tecniche, National Institute for Infectious Diseases "L. Spallanzani"-IRCCS, Rome, Italy
-
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani · Istituti di Ricovero e Cura a Carattere Scientifico
Affiliation as printed
Translational Research Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy
-
Affiliation as printed
Health Care and Preventive Medicine Unit, National Institute for Health, Migration and Poverty, Rome, Italy
-
Affiliation as printed
Health Care and Preventive Medicine Unit, National Institute for Health, Migration and Poverty, Rome, Italy
-
Affiliation as printed
UOC di Medicina e Rete Reumatologica, Ospedale Nuovo Regina Margherita, Rome, Italy
-
London School of Hygiene & Tropical Medicine
Affiliation as printed
Department of Infection Biology, London School of Hygiene and Tropical Medicine, London, United Kingdom
-
Simone A. Joosten Aachen
Leiden University · Leiden University Medical Center
Affiliation as printed
Leiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
-
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani · Istituti di Ricovero e Cura a Carattere Scientifico
Affiliation as printed
Translational Research Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy
-
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani
Affiliation as printed
Respiratory Infectious Diseases Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy
-
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani
Affiliation as printed
Respiratory Infectious Diseases Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy
-
Delia Goletti corresponding
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani · Istituti di Ricovero e Cura a Carattere Scientifico
Affiliation as printed
Translational Research Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, Rome, Italy. Electronic address: delia.goletti@inmi.it
Cited by 0 stored of 0
No patents citing this paper on Lens.org (checked 2026-10-11).