Efficient high-risk human papillomavirus screening through pooled testing: a simulation study based on real-world data in low- and middle-income countries
Journal of Global Health, vol. 16, pp. 04275
Abstract
Background: The World Health Organization recommends high-risk human papillomavirus (hrHPV) testing as the primary method for cervical cancer screening. Combining samples in a pool can reduce the expense associated with large numbers of commercially available tests. This study simulated the potential test-saving impact of pooled hrHPV testing using real-world data from two low- and middle-income countries (LMICs). Methods: We simulated a two- and three-stage hrHPV pooling in Bangladesh (hrHPV prevalence 2.10%) and Uganda (hrHPV prevalence 22.52%) using real-world laboratory data. Measurements included the number of tests per stage, the total number of tests, and the percentage of tests saved. We performed sensitivity analysis to assess the impact of a 1.00% loss of sensitivity and specificity per additional sample in the pool on the false-negative rate (FNR). Results: In Bangladesh, the two-stage strategy (eight samples per pool) and the three-stage strategy saved 71.21% and 77.21% of tests, respectively, compared to testing individually. In Uganda, the two-stage strategy (three samples per pool) reduced the number of tests by 15.34% compared to testing individually. Assuming loss of sensitivity and specificity due to pooling, the FNR was higher (FNR = 0.012-0.134) when hrHPV prevalence was low under the two-stage strategy. Conclusions: Pooled hrHPV testing has the potential to reduce the number of tests used and enhance screening capacity in resource-limited settings. Notably, a lower prevalence of hrHPV allows for more efficient pooled testing. Despite the potential loss of sensitivity and specificity, pooled hrHPV testing could offer benefits for LMICs and warrant further verification.
Authors 9
-
University Medical Center Groningen · University of Groningen
Affiliation as printed
Department of Epidemiology, University Medical Centre Groningen, University of Groningen, Groningen, the Netherlands
-
University Medical Center Groningen · University of Groningen
Affiliation as printed
Department of Radiology, University Medical Centre Groningen, University of Groningen, Groningen, the Netherlands
-
University Medical Center Groningen
Affiliation as printed
Unit of Global Health, Department of Health Sciences, University Medical Centre Groningen, Groningen, the Netherlands
-
Affiliation as printed
Global Health Academy, University of Edinburgh, Edinburgh, UK
-
Affiliation as printed
Health Sector, Friendship NGO, Dhaka, Bangladesh
-
Affiliation as printed
Department of Gynaecologic-Oncology, Uganda Cancer Institute, Kampala, Uganda
-
Leiden University · Leiden University Medical Center
Affiliation as printed
Department of Gynaecology, Leiden University Medical Centre, Leiden University, Leiden, the Netherlands
-
University Medical Center Groningen · University of Groningen
Affiliation as printed
Department of Epidemiology, University Medical Centre Groningen, University of Groningen, Groningen, the Netherlands
-
University Medical Center Groningen · University of Groningen · Open University of the Netherlands
Affiliation as printed
Department of Economics, Econometrics and Finance, University of Groningen, Groningen, the Netherlands
Faculty of Management Sciences, Open University, Heerlen, the Netherlands
Unit of Global Health, Department of Health Sciences, University Medical Centre Groningen, Groningen, the Netherlands
Cited by 0 stored of 0
No patents citing this paper on Lens.org (checked 2026-10-11).