Spatial transcriptomics defines the mechanisms of hiPSC-derived stem cell-mediated repair in human articular cartilage

bioRxiv (Cold Spring Harbor Laboratory)

Abstract

ABSTRACT We here determined therapeutic efficacy and mode-of-action of human induced pluripotent-derived therapeutic stem cells (hiMSCs) across in vivo mouse and ex vivo human osteoarthritis models. hiMSC treatment in DMM-mice significantly reduced OARSI damage scores, which was affirmed by a decrease in the catabolic marker Mmp13 and an increase in the anabolic marker Col2. These treatment effects appeared, irrespective of modifying factors such as xeno-free media or thermosensitive hydrogel carrier. Subsequently treatment of hiMSC+gel in human osteoarthritic cartilage explants showed a transcriptome-wide significant activation of the cholesterol and sterol synthesis pathways marked by genes such as MVD, DHCR7, MSMO1, FABP3 . Additionally, we showed that these changes alleviated OA-associated imbalances of the cellular Zinc-ion homeostasis pathways, represented by genes such as MT1F, MT1G, MT1H and SLC30A1 . Spatial transcriptomics then sensitively captured that hiMSC+gel treatment evoked, specifically at the superficial cartilage layer, a consistent upregulation of healthy chondrocyte markers such as CHAD, ACAN, FRZB , and SOX9 , alongside a suppression of catabolic and inflammatory mediators such as SERPINE1, SPP1, MMP13, ADAMTS5 . Our findings link therapeutic outcomes of hiMSC treatment to precise spatially resolved molecular changes in human tissue, that would otherwise be obscured by heterogeneous cell populations. Collectively our study highlighted that hiPSC-derived stem cell therapy (hiMSCs) could provide a scalable ‘off-the-shelf’ solution to treat osteoarthritis, with strong prospects for clinical applications in the near future.

Authors 13

1. Y.F. Ramos Aachen Dept Biomedical Data Sciences Section Molecular Epidemiology

   Leiden University Medical Center

   Affiliation as printed

   Department Biomedical Data Sciences, section Molecular Epidemiology, Leiden University Medical Center (LUMC), 2333 ZC Leiden, the Netherlands

2. Sayedeh Sana Sayedipour Aachen Dept Biomedical Data Sciences Section Molecular Epidemiology Dept. Radiology

   Leiden University Medical Center

   Affiliation as printed

   Department Biomedical Data Sciences, section Molecular Epidemiology, Leiden University Medical Center (LUMC), 2333 ZC Leiden, the Netherlands

   Department Radiology, LUMC, the Netherlands

3. Georgina Shaw

   Ollscoil na Gaillimhe – University of Galway

   Affiliation as printed

   Regenerative Medicine Institute, National University of Ireland Galway, Galway, Ireland

4. Margo Tuerlings Aachen Dept Biomedical Data Sciences Section Molecular Epidemiology

   Leiden University Medical Center

   Affiliation as printed

   Department Biomedical Data Sciences, section Molecular Epidemiology, Leiden University Medical Center (LUMC), 2333 ZC Leiden, the Netherlands

5. Timo Schomann Aachen Dept. Radiology

   Leiden University Medical Center

   Affiliation as printed

   Department Radiology, LUMC, the Netherlands

6. H. Eka D. Suchiman Aachen Dept Biomedical Data Sciences Section Molecular Epidemiology

   Leiden University Medical Center

   Affiliation as printed

   Department Biomedical Data Sciences, section Molecular Epidemiology, Leiden University Medical Center (LUMC), 2333 ZC Leiden, the Netherlands

7. Davy Cats Aachen Dept Biomedical Data Sciences

   Leiden University Medical Center

   Affiliation as printed

   Department Biomedical Data Sciences, Sequencing Analysis Support Core, LUMC, the Netherlands

8. Frank Barry

   Ollscoil na Gaillimhe – University of Galway

   Affiliation as printed

   Regenerative Medicine Institute, National University of Ireland Galway, Galway, Ireland

9. Rachid Mahdad Aachen Department Orthopaedics

   Alrijne Ziekenhuis

   Affiliation as printed

   Department Orthopaedics, Alrijne Hospital, Leiderdorp, the Netherlands

10. Hailiang Mei Aachen Dept Biomedical Data Sciences

    Leiden University Medical Center

    Affiliation as printed

    Department Biomedical Data Sciences, Sequencing Analysis Support Core, LUMC, the Netherlands

11. Luis Javier Cruz Aachen Dept. Radiology

    Leiden University Medical Center

    Affiliation as printed

    Department Radiology, LUMC, the Netherlands

12. Mary Murphy

    Ollscoil na Gaillimhe – University of Galway

    Affiliation as printed

    Regenerative Medicine Institute, National University of Ireland Galway, Galway, Ireland

13. Ingrid Meulenbelt corresponding Aachen Dept Biomedical Data Sciences Section Molecular Epidemiology

    Leiden University Medical Center

    Affiliation as printed

    Department Biomedical Data Sciences, section Molecular Epidemiology, Leiden University Medical Center (LUMC), 2333 ZC Leiden, the Netherlands