Synthesis_optimized_library.zip – FASTA and BED format files (2 × 27 files) with all proposed oligo probes targeting Brassica subgenome chromosomes using a maximum of 27k probes per chromosome

Zenodo (CERN European Organization for Nuclear Research)

Abstract

Synthesis_optimized_library.zip – FASTA and BED format files (2 × 27 files) with all proposed oligo probes targeting Brassica subgenome chromosomes using a maximum of 27k probes per chromosome targeting a similar density range (0.37 – 0.54 oligos per 1kb). There are painting probes for each of the 27 chromosomes (see Supplementary Table S1 for details). Both files per chromosome include similar information (chromosomal position on reference assemblies and nucleotide sequence). Used for commercial ordering. Figure S5 (Evaluation_ABC-genomes_synth_opt_lib.pdf): Localization of oligonucleotide sequences along chromosomes based on the B. rapa, B. nigra, and B. oleracea genomes. Chromosomes were divided into 100 kb windows, and the number of unique oligos recovered was calculated for each window. The distribution of the number of oligos is shown in the line plot. The x-axis is the position of the chromosome in megabase pair. The y-axis is the number of oligos in each 100 kb window.

Authors 6

1. Mariana Alejandra Baez

   University of Bonn

   Affiliation as printed

   University of Bonn

2. Ludwig Mann Aachen

   RWTH Aachen University

   Affiliation as printed

   RWTH Aachen University

3. Daniela Quezada-Martinez

   Justus-Liebig-Universität Gießen

   Affiliation as printed

   Justus-Liebig-Universität Gießen

4. Vinita Ramtekey

   Indian Council of Agricultural Research

   Affiliation as printed

   ICAR - Indian Institute of Seed Science

5. Tony Heitkam Aachen

   RWTH Aachen University

   Affiliation as printed

   RWTH Aachen University

6. Annaliese S. Mason

   University of Bonn

   Affiliation as printed

   University of Bonn