System and Method for En Masse Patterning of Molecule Structures

University of Chicago

Abstract

Devices, systems, and methods for en masse patterning of nucleic acid molecule structures are disclosed. The devices can include microchannels and nanoslits. The microchannels and nanoslits can be connected by parking chambers. The systems and methods can utilize the geometry of the devices in coordination with a voltage application routine to park nucleic acid molecules in the parking chambers and subsequently inject the nucleic acid molecules into the nanoslits. The methods can be utilized to present nucleic acid molecules in a fashion suitable for genomic analysis. The methods can also be utilized to provide size selection of the nucleic acid molecules.

Authors 7

1. David C. Schwartz

   University of Wisconsin–Madison

   Affiliation as printed

   University of Wisconsin-Madison

2. Kristy L. Kounovsky‐Shafer

   University of Wisconsin–Madison

   Affiliation as printed

   University of Wisconsin-Madison

3. Juan Pablo Hernandez-Ortiz

   University of Wisconsin–Madison

   Affiliation as printed

   University of Wisconsin-Madison

4. Konstantinos Dimitrios Potamousis

   University of Wisconsin–Madison

   Affiliation as printed

   University of Wisconsin-Madison

5. Juan Jose de Pablo

   University of Chicago

   Affiliation as printed

   University of Chicago

6. Theo Odijk Aachen

   Leiden University

   Affiliation as printed

   University of Leiden

7. Kyubong Jo

   University of Chicago

   Affiliation as printed

   University of Chicago