Data from: Environmental DNA metabarcoding effectively detects invasive species, pests, and community changes in Taiwan’s rice fields

DRYAD

Abstract

Rice fields represent man-made semi-aquatic wetlands primed for invasive pests. Monitoring rice field biodiversity using conventional methods, however, is time-consuming and laborious. Environmental DNA (eDNA) methods can provide a fast and effective means to monitor rice field communities and inform management decisions. Our study provides proof-of-concept of rice field eDNA biodiversity assessments, with a focus on native and non-native pests across cultivation phases. We collected eDNA samples from locations in southern Taiwan during planting and harvesting, employing eDNA metabarcoding (COI) to detect diverse taxonomic groups. We assigned 78 ASVs across all sites to animal taxa, 34 of which were identified to species. Overall, 18 species were designated as native or non-native (83.3 % and 16.6 %, respectively), including three major rice pests, Chilo suppressalis (native), Coptotermes formosanus (native), and Pomacea canaliculata (non-native). Cultivation affected overall diversity, with higher species richness during planting compared to harvesting. No significant differences were observed between native and non-native taxa between cultivation phases. Altogether, we detected a complex environment across trophic levels comprised of both native and non-native agricultural pests using limited sampling effort, demonstrating eDNA as an efficient biomonitoring approach in rice agroecosystems with direct applications for pest, invasive species, and vector surveillance within Taiwan.

Authors 10

1. Pritam Banerjee

   University of California, Berkeley

   Affiliation as printed

   University of California, Berkeley

2. Gobinda Dey

   National Chung Cheng University

   Affiliation as printed

   National Chung Cheng University

3. Kathryn A. Stewart Aachen

   Leiden University

   Affiliation as printed

   Leiden University

4. Matthew A. Barnes

   Texas Tech University

   Affiliation as printed

   Texas Tech University

5. Md. Taharia

   National Chung Cheng University

   Affiliation as printed

   National Chung Cheng University

6. Mathew Seymour

   University of Hong Kong

   Affiliation as printed

   University of Hong Kong

7. Chin-Wen Wang

   National Chung Cheng University

   Affiliation as printed

   National Chung Cheng University

8. Raju Kumar Sharma

   National Chung Cheng University

   Affiliation as printed

   National Chung Cheng University

9. Jyoti Prakash Maity

   KIIT University

   Affiliation as printed

   KIIT University

10. Chien-Yen Chen

    National Chung Cheng University

    Affiliation as printed

    National Chung Cheng University