Better reporting is better science: Community-defined minimal reporting requirements for light microscopy

The Journal of Cell Biology, vol. 225

Abstract

Incomplete reporting of microscopy methods undermines transparency, reproducibility, and data reuse. Despite recent initiatives, comprehensive, broadly endorsed, and accessible reporting guidelines are still lacking. Here, we present a bare minimal microscopy reporting requirements checklist that integrates human- and machine-readable input to provide clear, actionable guidance for researchers, reviewers, and publishers and to advance community standards in microscopy.

Authors 42

1. Paula D.L.M. Montero Llopis

   Harvard University

   Affiliation as printed

   MicRoN Core Facility, Harvard Medical School 1 , Boston, MA,

2. Chloë van Oostende‐Triplet

   University of Ottawa

   Affiliation as printed

   CBIA Core Facility, Faculty of Medicine, University of Ottawa 2 , Ottawa,

3. Nathalie Gaudreault

   Allen Institute for Cell Science

   Affiliation as printed

   Allen Institute for Cell Science 3 , Seattle, WA,

4. Caterina Strambio‐De‐Castillia

   University of Massachusetts Chan Medical School

   Affiliation as printed

   Program in Molecular Medicine, UMass Chan Medical School, Worcester, MA, USA 4

5. Julia Fernandez-Rodriguez

   University of Gothenburg

   Affiliation as printed

   Centre for Cellular Imaging, University of Gothenburg 5 , Gothenburg,

6. Gabriel G. Martins

   Gulbenkian Institute for Molecular Medicine

   Affiliation as printed

   Gulbenkian Institute for Molecular Medicine 6 , Lisbon,

7. Alison J. North

   Rockefeller University

   Affiliation as printed

   Bio-Imaging Resource Center, The Rockefeller University 7 , New York, NY,

8. Luis Acevedo
   Affiliation as printed

   Quantum Medical Diagnostics & UMKC 8 , Overland Park, KS,

9. Sergiy Avilov

   Max Planck Institute of Immunobiology and Epigenetics

   Affiliation as printed

   Max Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany 9

10. Cristina Bertocchi

    Pontificia Universidad Católica de Chile · The University of Osaka

    Affiliation as printed

    Pontificia Universidad Católica de Chile 10 , Santiago,

    University of Osaka 11 , Osaka,

11. Ulrike Boehm

    Carl Zeiss (Germany)

    Affiliation as printed

    Carl Zeiss AG 12 , Oberkochen,

12. Michael Cammer

    NYU Langone Health · New York University

    Affiliation as printed

    Microscopy Core, NYU Langone Grossman School of Medicine 14 , New York, NY,

13. Aurélie Cleret‐Buhot

    Université de Montréal

    Affiliation as printed

    Research Center of the Hospital, Center of Université de Montréal (CRCHUM) 15 , Montréal,

14. Steffen Dietzel

    Institut für Kardiovaskuläre Physiologie und Pathophysiologie · Ludwig-Maximilians-Universität München

    Affiliation as printed

    Core Facility Bioimaging and Institut für Kardiovaskuläre Physiologie und Pathophysiologie, Biomedical Center, Ludwig-Maximilians-Universität München, Munich Germany 16

15. Orestis Faklaris

    Centre National de la Recherche Scientifique · Université Paris Cité · Institut Jacques Monod

    Affiliation as printed

    Institut Jacques Monod, Université Paris Cité, CNRS 17 , Paris,

16. David C. A. Gaboriau

    Imperial College London

    Affiliation as printed

    Facility for Imaging by Light Microscopy (FILM), Imperial College London 18 , London,

17. Thomas Guilbert
    Affiliation as printed

    INSCOPER 19 , Cesson-Sévigné,

18. Nadia Halidi

    Barcelona Institute of Science and Technology · Centre for Genomic Regulation

    Affiliation as printed

    Advanced Light Microscopy Unit, Centre for Genomic Regulation (CRG) 22 , Barcelona,

    The Barcelona Institute of Science and Technology 23 , Barcelona,

19. Mathias Hammer

    University of Massachusetts Chan Medical School

    Affiliation as printed

    RNA Therapeutics Institute, University of Massachusetts Chan Medical School 20 , Worcester, MA,

20. Hella Hartmann

    Center for Molecular and Cellular Bioengineering · Technische Universität Dresden

    Affiliation as printed

    CMCB Technology Platform, Light Microscopy Facility, TU Dresden 24 , Dresden,

21. Helena Klara Jambor

    University of Applied Sciences of the Grisons

    Affiliation as printed

    DAViS, University of Applied Sciences of the Grisons 27 , Chur,

22. Ayse Aslihan Koksoy

    The University of Texas MD Anderson Cancer Center

    Affiliation as printed

    UT MD Anderson Cancer Center 28 , Houston, TX,

23. Judith Lacoste

    MIA Cellavie Inc. (Canada)

    Affiliation as printed

    MIA Cellavie Inc. 29 , Montreal,

24. DeLaine D. Larsen
    Affiliation as printed

    Eikon Therapeutics 30 , Hayward, CA,

25. Sylvia E. Le Dévédec Aachen Leiden Academic Centre for Drug Research

    Leiden University

    Affiliation as printed

    Leiden Academic Centre for Drug Research (LACDR), Leiden University, Leiden, the Netherlands 31

26. Penghuan Liu

    China Jiliang University

    Affiliation as printed

    College of Optical and Electronic Technology, China Jiliang University 32 , Hangzhou,

27. Josh Moore

    German BioImaging – Gesellschaft für Mikroskopie und Bildanalyse

    Affiliation as printed

    German BioImaging-Gesellschaft für Mikroskopie und Bildanalyse e.V. 33 , Constance,

28. Glyn Nelson

    Newcastle University

    Affiliation as printed

    Bioimaging Unit, Newcastle University 34 , Newcastle Upon Tyne,

29. Nils Norlin

    Lund University · NanoLund

    Affiliation as printed

    Lund University 37 Department of Experimental Medical Science, , Lund,

    Lund University bioimaging Centre, Lund University 36 , Lund,

    NanoLund, Lund University 38 , Lund,

30. Adam C. Parslow

    Baker Heart and Diabetes Institute

    Affiliation as printed

    Baker Heart and Diabetes Institute 39 , Melbourne,

31. Alex L. Payne-Dwyer

    University of York

    Affiliation as printed

    University of York 40 Bioscience Technology Facility, Department of Biology, , York,

32. John W. Peterson

    Cleveland Clinic

    Affiliation as printed

    Imaging Core, Shared Laboratory Resources, Lerner Research Institute, Cleveland Clinic 41 , Cleveland, OH,

33. Santosh Podder

    Indian Institute of Science Education and Research Pune

    Affiliation as printed

    Microscopy Facility, IISER 42 , Pune,

34. Andrea Ravasio

    Pontificia Universidad Católica de Chile

    Affiliation as printed

    Institute for Biological and Medical Engineering (IIBM) Schools of Engineering, Medicine and Biological Sciences Pontificia Universidad Católica de Chile, Santiago, Chile 43

35. Eduardo Rosa‐Molinar

    Washington University in St. Louis

    Affiliation as printed

    Washington University in Saint Louis School of Medicine, Washington University Center for Cellular Imaging, St. Louis, MO, USA 44 Department of Cell Biology & Physiology and Neuroscience,

36. Britta Schroth‐Diez

    Max Planck Institute of Molecular Cell Biology and Genetics

    Affiliation as printed

    Light Microscopy Facility, Max Planck Institute of Molecular Cell Biology and Genetics 45 , Dresden,

37. Olaf Selchow
    Affiliation as printed

    Microscopy & BioImaging Consulting, Thuringia, Germany 46

38. Sathya Srinivasan

    Oregon National Primate Research Center

    Affiliation as printed

    Imaging and Morphology Support Core - Integrated Pathology Core, Oregon National Primate Research Center 47 , Beaverton, OR,

39. Douglas J. Taatjes

    University of Vermont

    Affiliation as printed

    Microscopy Imaging Center. Larner College of Medicine, University of Vermont 48 , Burlington, VT,

40. Kirstin Vonderstein

    University of Helsinki

    Affiliation as printed

    Biomedicum Imaging Unit (BIU), University of Helsinki 49 , Helsinki,

41. Christa G Walther

    German BioImaging – Gesellschaft für Mikroskopie und Bildanalyse

    Affiliation as printed

    GermanBioImaging 50 , Konstanz,

    GloBIAS 51 , Klosterneuburg,

42. Roland Nitschke

    University of Freiburg

    Affiliation as printed

    Life Imaging Center and Signalling Research Centres CIBSS and BIOSS, University of Freiburg 52 , Freiburg,