Intelligent image-activated sorting of large cells enabled by elasto-inertial focusing

Lab on a Chip, vol. 26, pp. 2047–2060

Abstract

, a large microalgal cell species, based on intracellular lipid droplet formation, demonstrating 4.5-fold enrichment of target cells from 11.9% to 53.8% at 128 eps. Our work highlights that IACS based on elasto-inertial focusing enables the sorting of large objects based on high-content real-time image analysis without compromising sorting purity, yield, or event rate.

Authors 10

1. Yuzuki Nagasaka

   The University of Tokyo

   Affiliation as printed

   Department of Chemistry, Graduate School of Science, The University of Tokyo, Tokyo 113-0033, Japan

2. Akihiro Isozaki

   Ritsumeikan University · The University of Tokyo

   Affiliation as printed

   Department of Chemistry, Graduate School of Science, The University of Tokyo, Tokyo 113-0033, Japan

   Department of Mechanical Engineering, College of Science and Engineering, Ritsumeikan University, Shiga 525-8577, Japan

3. Hiroki Matsumura

   Georgia Institute of Technology · The University of Tokyo

   Affiliation as printed

   Department of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30322, USA

   Department of Chemistry, Graduate School of Science, The University of Tokyo, Tokyo 113-0033, Japan

4. Natsumi Tiffany Ishii

   The University of Tokyo

   Affiliation as printed

   Department of Chemistry, Graduate School of Science, The University of Tokyo, Tokyo 113-0033, Japan

5. Norah Roels

   The University of Tokyo

   Affiliation as printed

   Department of Chemistry, Graduate School of Science, The University of Tokyo, Tokyo 113-0033, Japan

6. Mina Rassuli Aachen Department of Chemistry

   RWTH Aachen University

   Affiliation as printed

   Department of Chemistry, RWTH Aachen University, Aachen 52074, Germany

7. Kelvin C. M. Lee

   The University of Tokyo

   Affiliation as printed

   Department of Chemistry, Graduate School of Science, The University of Tokyo, Tokyo 113-0033, Japan

8. Walker Peterson

   The University of Tokyo

   Affiliation as printed

   Department of Chemistry, Graduate School of Science, The University of Tokyo, Tokyo 113-0033, Japan

9. Tianben Ding corresponding

   The University of Tokyo

   Affiliation as printed

   Department of Chemistry, Graduate School of Science, The University of Tokyo, Tokyo 113-0033, Japan

10. Goda Keisuke corresponding

    Tohoku University · Wuhan University · The University of Tokyo

    Affiliation as printed

    Department of Chemistry, Graduate School of Science, The University of Tokyo, Tokyo 113-0033, Japan

    Graduate School of Medicine, Tohoku University, Miyagi 980-0872, Japan

    Institute of Technological Sciences, Wuhan University, Wuhan, Hubei 430072, China

    International Centre for Synchrotron Radiation Innovation Smart, Tohoku University, Miyagi 980-8572, Japan

    SiRIUS Institute of Medical Research, Tohoku University, Miyagi 980-0872, Japan