Photoemission electron microscopy for connectomics

Proceedings of the National Academy of Sciences, vol. 122, pp. e2521349122

Abstract

Photoemission electron microscopy (PEEM) offers a potential third modality for large-volume connectomics alongside transmission electron microscopy (TEM) and scanning electron microscopy (SEM). We image osmium stained, ultrathin brain sections on gold coated silicon at synaptic resolution using commercial PEEMs. At coarser resolution, we demonstrate that ultraviolet laser illumination enables gigavoxel-per-second acquisition rates without thermal damage. PEEM combines TEM-like parallel detection with SEM-compatible solid supports into a potentially scalable and cost-effective approach for large-volume connectomes.

Authors 16

1. Gregg A. Wildenberg corresponding

   Argonne National Laboratory · University of Chicago

   Affiliation as printed

   Biosciences Division, Argonne National Laboratory

   Department of Neurobiology, University of Chicago

   Biosciences Division, Argonne National Laboratory, Lemont, IL 60439

   Department of Neurobiology, University of Chicago, Chicago, IL 60637

2. Kevin M. Boergens corresponding

   University of Illinois Chicago

   Affiliation as printed

   Department of Physics, University of Illinois Chicago

   Department of Physics, University of Illinois Chicago, Chicago, IL 60607

3. Lola Lambert corresponding

   University of Chicago

   Affiliation as printed

   Department of Neurobiology, University of Chicago

   Department of Neurobiology, University of Chicago, Chicago, IL 60637

4. Ruiyu Li

   University of Chicago

   Affiliation as printed

   Department of Chemistry, University of Chicago

   James Franck Institute, University of Chicago

   Department of Chemistry, University of Chicago, Chicago, IL 60637

5. Michael K. L. Man

   Okinawa Institute of Science and Technology Graduate University

   Affiliation as printed

   Okinawa Institute of Science and Technology, Femtosecond Spectroscopy Unit

   Okinawa Institute of Science and Technology, Femtosecond Spectroscopy Unit, Onna-son, Kunigami-gun Okinawa 904-0495, Japan

6. Amin Moradi Aachen

   Leiden University

   Affiliation as printed

   Leiden Institute of Physics, Leiden University

   Leiden Institute of Physics, Leiden University, Leiden 2333 CA, The Netherlands

7. Janek Rieger

   University of Chicago

   Affiliation as printed

   Department of Chemistry, University of Chicago

   James Franck Institute, University of Chicago

   Department of Chemistry, University of Chicago, Chicago, IL 60637

8. Hengli Duan

   Diamond Light Source

   Affiliation as printed

   Diamond Light Source, Magnetic Materials Group

   Diamond Light Source, Magnetic Materials Group, Didcot OX110DE, United Kingdom

9. Sarnjeet S. Dhesi

   Diamond Light Source

   Affiliation as printed

   Diamond Light Source, Magnetic Materials Group

   Diamond Light Source, Magnetic Materials Group, Didcot OX110DE, United Kingdom

10. Γαβριήλ Καρράς

    Diamond Light Source

    Affiliation as printed

    Diamond Light Source, Magnetic Materials Group

    Diamond Light Source, Magnetic Materials Group, Didcot OX110DE, United Kingdom

11. Francesco Maccherozzi

    Diamond Light Source

    Affiliation as printed

    Diamond Light Source, Magnetic Materials Group

    Diamond Light Source, Magnetic Materials Group, Didcot OX110DE, United Kingdom

12. Keshav M. Dani

    Okinawa Institute of Science and Technology Graduate University

    Affiliation as printed

    Okinawa Institute of Science and Technology, Femtosecond Spectroscopy Unit

    Okinawa Institute of Science and Technology, Femtosecond Spectroscopy Unit, Onna-son, Kunigami-gun Okinawa 904-0495, Japan

13. Rudolf M. Tromp Aachen

    Leiden University

    Affiliation as printed

    Leiden Institute of Physics, Leiden University

    Leiden Institute of Physics, Leiden University, Leiden 2333 CA, The Netherlands

14. Sense Jan van der Molen Aachen

    Leiden University

    Affiliation as printed

    Leiden Institute of Physics, Leiden University

    Leiden Institute of Physics, Leiden University, Leiden 2333 CA, The Netherlands

15. Sarah B. King corresponding

    University of Chicago

    Affiliation as printed

    Department of Chemistry, University of Chicago

    James Franck Institute, University of Chicago

    Department of Chemistry, University of Chicago, Chicago, IL 60637

16. Narayanan Kasthuri corresponding

    Argonne National Laboratory · University of Chicago

    Affiliation as printed

    Biosciences Division, Argonne National Laboratory

    Department of Neurobiology, University of Chicago

    Biosciences Division, Argonne National Laboratory, Lemont, IL 60439

    Department of Neurobiology, University of Chicago, Chicago, IL 60637