Reperfused human umbilical cords as an ex vivo model for ECMO cannulation in artificial placenta technology

Frontiers in Bioengineering and Biotechnology, vol. 13, pp. 1663442

Abstract

Background: Establishing an extracorporeal circuit for artificial placenta technologies requires the cannulation of the umbilical vessels with large-bore cannulas. So far, this procedure could only be performed in large-animal models. We present the methodology and a novel test setup for reperfusion of human umbilical cords to develop and train cannulation procedures. Methods: 67 umbilical cords and placentas were harvested during cesarian sections, 15 cm umbilical cord specimen and fetal blood were used for these experiments. All three umbilical vessels were flushed, terminal cannulated, and connected to a circuit. We performed systematic validation tests on reperfusion of 15 umbilical cord specimens, followed by an ECMO cannulation of all three reperfused vessels. Results: The terminal cannulation succeeded in 43 of 67 cases. 15 of these were included in the validation study. A physiological flow of 71.2 mL/min ±3.9 mL/min (mean ± SD) could be established. ECMO Cannulation of at least one artery and one vein succeeded in 9 of 15 cases, and cannulation of all three vessels in five cases. Conclusion: Reperfusion of human umbilical cord specimens with fetal blood can provide a biosimilar model for the development, testing, and training of umbilical vessel cannulation with large-bore cannulas.

Authors 7

1. Camila Hoyos Banchon corresponding Aachen Faculty of Medicine Neonatology Section Pediatric Clinic

   RWTH Aachen University · Universitätsklinikum Aachen

   Affiliation as printed

   Pediatric Clinic, Neonatology Section, Faculty of Medicine, RWTH Aachen University and University Hospital, Aachen, Germany

2. Jan Heyer Aachen Institute of Applied Medical Engineering Department of Cardiovascular Engineering

   RWTH Aachen University · Universitätsklinikum Aachen

   Affiliation as printed

   Department of Cardiovascular Engineering, Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University and University Hospital, Faculty of Medicine, Aachen, Germany

3. Juliette S. van Haren

   Eindhoven University of Technology

   Affiliation as printed

   Department of Industrial Design, Eindhoven University of Technology, Eindhoven, Netherlands

4. Thorsten W Orlikowsky Aachen Institute of Applied Medical Engineering Department of Cardiovascular Engineering

   RWTH Aachen University · Universitätsklinikum Aachen

   Affiliation as printed

   Department of Cardiovascular Engineering, Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University and University Hospital, Faculty of Medicine, Aachen, Germany

5. Ulrich Steinseifer Aachen Institute of Applied Medical Engineering Department of Cardiovascular Engineering

   RWTH Aachen University · Universitätsklinikum Aachen

   Affiliation as printed

   Department of Cardiovascular Engineering, Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University and University Hospital, Faculty of Medicine, Aachen, Germany

6. Sebastian Victor Jansen Aachen Institute of Applied Medical Engineering Department of Cardiovascular Engineering

   RWTH Aachen University · Universitätsklinikum Aachen

   Affiliation as printed

   Department of Cardiovascular Engineering, Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University and University Hospital, Faculty of Medicine, Aachen, Germany

7. Mark Schoberer Aachen Institute of Applied Medical Engineering Department of Cardiovascular Engineering

   RWTH Aachen University · Universitätsklinikum Aachen

   Affiliation as printed

   Department of Cardiovascular Engineering, Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University and University Hospital, Faculty of Medicine, Aachen, Germany