Release, Transfer, Fold: Using a Silicone Adhesive for On-Demand 3D Tissue Engineering
ACS Biomaterials Science & Engineering, vol. 11, pp. 6506–6514
Abstract
Conventional cell culture substrates are flat and rigid, locking cells in a permanent and unphysiological geometry. Advanced tissue culture models that emulate the dynamic and 3D environments of organs remain challenging to generate. Here, we establish flexible silicone adhesive films as versatile substrates that enable the on-demand release, transfer, and folding of cultured 2D tissues into 3D geometries. We rolled primary epithelial cultures into tubes, assembled cuboidal structures, and transferred primary endothelial cultures between culture environments for coculturing. Our approach provides an easy-to-implement platform for dynamic geometrical designs in tissue engineering.
Authors 7
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Helmholtz Munich · German Center for Lung Research · Bioengineering Center · Institute of Biological and Medical Imaging · Technical University of Munich
Affiliation as printed
Chair of Biological Imaging at the Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine and Health
Comprehensive Pneumology Center Munich
German Center for Lung Research (DZL)
Helmholtz Pioneer Campus
Helmholtz Zentrum München
Institute of Biological and Medical Imaging, Bioengineering Center
Technical University of Munich
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Heidelberg University · Helmholtz Munich · German Center for Lung Research · Bioengineering Center · Institute of Biological and Medical Imaging · Technical University of Munich
Affiliation as printed
Chair of Biological Imaging at the Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine and Health
Comprehensive Pneumology Center Munich
Faculty of Engineering Sciences
German Center for Lung Research (DZL)
Helmholtz Pioneer Campus
Helmholtz Zentrum München
Institute of Biological and Medical Imaging, Bioengineering Center
Technical University of Munich
University of Heidelberg
Chair of Biological Imaging at the Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine and Health, Technical University of Munich, Munich, Munich D-81675, Germany
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Helmholtz Munich · German Center for Lung Research · Bioengineering Center · Institute of Biological and Medical Imaging · Technical University of Munich
Affiliation as printed
Chair of Biological Imaging at the Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine and Health
Comprehensive Pneumology Center Munich
German Center for Lung Research (DZL)
Helmholtz Pioneer Campus
Helmholtz Zentrum München
Institute of Biological and Medical Imaging, Bioengineering Center
Technical University of Munich
-
Helmholtz Munich · German Center for Lung Research · Bioengineering Center · Institute of Biological and Medical Imaging · Technical University of Munich
Affiliation as printed
Chair of Biological Imaging at the Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine and Health
Comprehensive Pneumology Center Munich
German Center for Lung Research (DZL)
Helmholtz Pioneer Campus
Helmholtz Zentrum München
Institute of Biological and Medical Imaging, Bioengineering Center
Technical University of Munich
-
Helmholtz Munich · German Center for Lung Research · Bioengineering Center · Institute of Biological and Medical Imaging · Technical University of Munich
Affiliation as printed
Chair of Biological Imaging at the Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine and Health
Comprehensive Pneumology Center Munich
German Center for Lung Research (DZL)
Helmholtz Pioneer Campus
Helmholtz Zentrum München
Institute of Biological and Medical Imaging, Bioengineering Center
Technical University of Munich
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Anne M. van der Does Aachen
Leiden University Medical Center
Affiliation as printed
Leiden University Medical Center
PulmoScience Lab, Department of Pulmonology
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Janna C. Nawroth corresponding
Helmholtz Munich · German Center for Lung Research · Bioengineering Center · Institute of Biological and Medical Imaging · Technical University of Munich
Affiliation as printed
Chair of Biological Imaging at the Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine and Health
Comprehensive Pneumology Center Munich
German Center for Lung Research (DZL)
Helmholtz Pioneer Campus
Helmholtz Zentrum München
Institute of Biological and Medical Imaging, Bioengineering Center
Technical University of Munich
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